Today, through lab-on-chip, microfluidics has become an essential element of biology.
The BioMicrofluidics unit is naturally integrated with the entire range of IEMN platforms. From the design of micro-devices, to their fabrication and characterization, to their use in biology in the BioMicrofluidics laboratory.
The BioMicrofluidics unit is composed of a BioMicrofluidics area offering in the same space an L2 biology laboratory associated with a set of microfluidic equipment, as well as a Soft Lithography area.
Contact:
Aude Sivery,
Technical manager of the platform
03 20 19 19 17
aude.sivery@univ-lille.fr
Bio Micro Fluidic Resource
The Bio/microfluidic area, designed in compliance with the safety standards of an L2 biology laboratory, offers all the equipment necessary for cell biology (type II PSM, control microscope, CO2 chamber, etc.). We also have all the consumables dedicated to cell culture (pipettes, flasks, eppendorfs...). An autoclave allowing the inactivation of waste, is also used for the sterilization of micro-devices. In this area, there are also work surfaces equipped with fluidic actuation systems: syringe pump, pressure controller, flow meters... for the study of micro-devices in flow. On each of these work areas, a CO2 incubator is available to allow cell culture in microsystems.
This space also offers a microscopy room with two microscopes. These microscopes allow phase contrast and epi-fluorescence imaging. The 1st microscope is an Olympus IX 71, it is equipped with a heating and motorized X, Y stage as well as a high sensitivity fast camera. The 2nd microscope is a Leica DMi8 with a motorized X, Y and Z stage. It allows to make long time lapse thanks to the adaptive focus holding system and its environment chamber (temperature and CO2 concentration control). This microscope is particularly adapted to real-time monitoring of cells in fluidic microsystems. A photopatterning system (Primo from Alvéole) is also installed on the Leica DMi8. It allows to do protein micropatterning and to generate in a few seconds any pattern with several proteins on classical cell culture media.
The platform recently acquired a 3D bioprinter. Thanks to the 3D bioprinting technique, cells and biomaterials can be deposited layer by layer to create cellular structures with properties close to natural tissues.
The BioMicrofluidics platform is open to internal and external academic projects. It is also open to industrial projects (collaboration or service). We offer training in cell culture, in handling microfluidic equipment and also in microscopy. The rates for these services are available here.
Soft-Lithography Resource
Creation of a new resource as an integral part of the CMNF, for the development and characterization of microfluidic devices
- Within this resource, a digital milling machine control placed in the back-end allows the machining of fluidic components (chips in particular), or mechanical (mold for example) in polymers or other hard materials
- A workstation dedicated to the manufacture of polymers (PDMS mainly) includes all the necessary equipment for the formulation of polymer mixtures (planetary mixer, precision balance, degasser, oven, spin-coater...)
- Thanks to the PICO plasma frame, cleaning as well as surface activation is possible
- A station dedicated to xurography will allow the cutting of thin polymer films with a tracing knife
- A KRUSS goniometer is also available to allow surface characterization for wettability and contact angle type measurements
- Finally, a station dedicated to the control of chips at the end of the process is equipped with a pressure controller as well as a stereoscopic microscope for device observationFinally, a station dedicated to the control of chips at the end of the process is equipped with a pressure controller as well as a stereoscopic microscope for device observation
Creation of a technological and scientific continuity between the soft-lithography resource and the Bio-Microfluidics laboratory
Contact:
Aude Sivery
Technical manager of the platform
03 20 19 19 17
aude.sivery@univ-lille.frJérôme Follet
Scientific manager of the platform
jerome.follet@univ-lille.fr
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