{"id":75353,"date":"2025-10-09T08:21:04","date_gmt":"2025-10-09T06:21:04","guid":{"rendered":"https:\/\/www.iemn.fr\/?p=75353"},"modified":"2025-10-09T08:21:41","modified_gmt":"2025-10-09T06:21:41","slug":"seminaire-prof-yutetsu-kuruma-october-20-2025-construction-of-artificial-cells-capable-of-self-reproduction-and-its-application","status":"publish","type":"post","link":"https:\/\/www.iemn.fr\/en\/a-la-une\/seminaire-prof-yutetsu-kuruma-october-20-2025-construction-of-artificial-cells-capable-of-self-reproduction-and-its-application.html","title":{"rendered":"S\u00e9minaire : Prof. Yutetsu Kuruma \/ October 20, 2025: Construction of artificial cells capable of self-reproduction and its application"},"content":{"rendered":"<div  class='flex_column av-av_one_full-ffd32c5c0f45105b246d723872274e0c av_one_full  avia-builder-el-0  avia-builder-el-no-sibling  first flex_column_div'     ><section  class='av_textblock_section av-mgj13q1o-9846c682cd764e2518d88df0e27203ab'   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><h3 style=\"text-align: center;\">Seminar<\/h3>\n<p style=\"text-align: center;\"><strong><span style=\"color: #008080;\">Prof. Yutetsu Kuruma<\/span><br \/>\n<\/strong><em>from the Japan Agency for Marine-Earth Science and Technology (JAMSTEC)<\/em><br \/>\nOctober 20, 2025 &#8211; 2:00 PM \/ IEMN &#8211; Amphitheater<\/p>\n<h3 style=\"text-align: center;\"><span style=\"color: #008080;\">Construction of artificial cells capable of self-reproduction and its application<\/span><\/h3>\n<hr \/>\n<p><strong>Abstract:<\/strong><br \/>\nArtificial cell research\u2014rebuilding minimal cells from defined molecules and genes\u2014leads a constructive understanding of biological systems and provides hints to soluving the mystery of the origin of life. By encapsulating reaction networks in cell-sized phospholipid vesicles, functions such as protein synthesis, energy generation, and even division-like events have been reconstituted. However, autonomous self-replication, which is the most remarkable feature of life, remains challenge because sustained membrane growth by assembling molecules has been difficult to achieve. We have constructed a lipid-synthesizing artificial cell by assembling a cell-free protein synthesis system, fatty acid synthesis system, and an acetyl-CoA\u2013generating module, that achieved de novo phospholipids (phosphatidic acids) synthesis within the membrane vesicles. In vitro quantification showed almost 100 \u00b5M phospholipid produced inside. Because 1 mM is required to double membrane surface area, we are redesigning and developing the system to increase productivity. Especially, we are focusing on NADH supply\u2014the key cofactor for fatty-acid synthesis\u2014by coupling the network to a lactate degradation pathway to create an NADH self-replenishing artificial cell. Furthermore, as application study, we developed a cell-free platform in which antibodies synthesized in cell-free system are displayed on 100 nm liposome membranes, producing immunoliposomes that bind selectively to target cancer cells. This approach can be used as a technology for drug delivery and gene transfer.<\/p>\n<\/div><\/section><\/div>","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":69379,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3,8],"tags":[],"class_list":["post-75353","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-a-la-une","category-actualites"],"_links":{"self":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts\/75353","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/comments?post=75353"}],"version-history":[{"count":2,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts\/75353\/revisions"}],"predecessor-version":[{"id":75705,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts\/75353\/revisions\/75705"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/media\/69379"}],"wp:attachment":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/media?parent=75353"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/categories?post=75353"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/tags?post=75353"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}