{"id":75218,"date":"2025-08-23T08:39:35","date_gmt":"2025-08-23T06:39:35","guid":{"rendered":"https:\/\/www.iemn.fr\/?p=75218"},"modified":"2025-09-23T08:53:39","modified_gmt":"2025-09-23T06:53:39","slug":"these-shakila-behzadifar-nanoparticules-organiques-pour-le-ciblage-des-cellules-%ce%b2-pancreatiques-dans-le-diabete-de-type-1-et-de-type-2","status":"publish","type":"post","link":"https:\/\/www.iemn.fr\/en\/a-la-une\/these-shakila-behzadifar-nanoparticules-organiques-pour-le-ciblage-des-cellules-%ce%b2-pancreatiques-dans-le-diabete-de-type-1-et-de-type-2.html","title":{"rendered":"THESE : Shakila BEHZADIFAR &#8211; 24\/09\/2025 &#8211; Nanoparticules organiques pour le ciblage des cellules \u03b2 pancr\u00e9atiques dans le diab\u00e8te de type 1 et de type 2"},"content":{"rendered":"<div id='layer_slider_1'  class='avia-layerslider main_color avia-shadow  avia-builder-el-0  el_before_av_heading  avia-builder-el-first  container_wrap sidebar_right'  style='height: 261px;'  ><div id=\"layerslider_58_1xhwn0ns8qia7\" data-ls-slug=\"homepageslider\" class=\"ls-wp-container fitvidsignore ls-selectable\" style=\"width:1140px;height:260px;margin:0 auto;margin-bottom: 0px;\"><div class=\"ls-slide\" data-ls=\"duration:6000;transition2d:5;\"><img loading=\"lazy\" decoding=\"async\" width=\"2600\" height=\"270\" src=\"https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1.jpg\" class=\"ls-bg\" alt=\"\" srcset=\"https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1.jpg 2600w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1-300x31.jpg 300w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1-768x80.jpg 768w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1-1030x107.jpg 1030w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1-1500x156.jpg 1500w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1-705x73.jpg 705w\" sizes=\"auto, (max-width: 2600px) 100vw, 2600px\" \/><ls-layer style=\"font-size:14px;text-align:left;font-style:normal;text-decoration:none;text-transform:none;font-weight:700;letter-spacing:0px;border-style:solid;border-color:#000;background-position:0% 0%;background-repeat:no-repeat;width:180px;height:30px;left:0px;top:231px;line-height:32px;color:#ffffff;border-radius:6px 6px 6px 6px;padding-left:50px;background-color:rgba(0, 0, 0, 0.57);\" class=\"ls-l ls-ib-icon ls-text-layer\" data-ls=\"minfontsize:0;minmobilefontsize:0;\"><i class=\"fa fa-quote-right\" style=\"color:#ffffff;margin-right:0.8em;font-size:1em;transform:translateY( -0.125em );\"><\/i>ACTUALITES<\/ls-layer><\/div><\/div><\/div><div id='after_layer_slider_1'  class='main_color av_default_container_wrap container_wrap sidebar_right'  ><div class='container av-section-cont-open' ><div class='template-page content  av-content-small alpha units'><div class='post-entry post-entry-type-page post-entry-75218'><div class='entry-content-wrapper clearfix'>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-k5dohoxw-658483151cc4b14c9a819415f8dd9337\">\n#top .av-special-heading.av-k5dohoxw-658483151cc4b14c9a819415f8dd9337{\nmargin:0 0 10px 0;\npadding-bottom:4px;\n}\nbody .av-special-heading.av-k5dohoxw-658483151cc4b14c9a819415f8dd9337 .av-special-heading-tag .heading-char{\nfont-size:25px;\n}\n.av-special-heading.av-k5dohoxw-658483151cc4b14c9a819415f8dd9337 .av-subheading{\nfont-size:15px;\n}\n<\/style>\n<div  class='av-special-heading av-k5dohoxw-658483151cc4b14c9a819415f8dd9337 av-special-heading-h2  avia-builder-el-1  el_after_av_layerslider  el_before_av_hr  avia-builder-el-first  av-linked-heading'><h2 class='av-special-heading-tag'  itemprop=\"headline\"  >THESE : Shakila BEHZADIFAR &#8211; Nanoparticules organiques pour le ciblage des cellules \u03b2 pancr\u00e9atiques dans le diab\u00e8te de type 1 et de type 2<\/h2><div class=\"special-heading-border\"><div class=\"special-heading-inner-border\"><\/div><\/div><\/div>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-18u73nj-dad6a947580930e400fc42ba200e80f1\">\n#top .hr.av-18u73nj-dad6a947580930e400fc42ba200e80f1{\nmargin-top:5px;\nmargin-bottom:5px;\n}\n.hr.av-18u73nj-dad6a947580930e400fc42ba200e80f1 .hr-inner{\nwidth:100%;\n}\n<\/style>\n<div  class='hr av-18u73nj-dad6a947580930e400fc42ba200e80f1 hr-custom  avia-builder-el-2  el_after_av_heading  el_before_av_textblock  hr-left hr-icon-no'><span class='hr-inner inner-border-av-border-thin'><span class=\"hr-inner-style\"><\/span><\/span><\/div>\n<section  class='av_textblock_section av-jriy64i8-fd5f2e9d63bf552d6910d12f255eb26e'   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" >\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-13ewzjw-68e036126b913e5028f77311dc66b825\">\n.av_font_icon.av-13ewzjw-68e036126b913e5028f77311dc66b825{\ncolor:#bfbfbf;\nborder-color:#bfbfbf;\n}\n.av_font_icon.av-13ewzjw-68e036126b913e5028f77311dc66b825 .av-icon-char{\nfont-size:60px;\nline-height:60px;\n}\n<\/style>\n<span  class='av_font_icon av-13ewzjw-68e036126b913e5028f77311dc66b825 avia_animate_when_visible av-icon-style- avia-icon-pos-left avia-icon-animate'><span class='av-icon-char' aria-hidden='true' data-av_icon='\ue8c9' data-av_iconfont='entypo-fontello' ><\/span><\/span>\n<p><strong>Shakila BEHZADIFAR<\/strong><\/p>\n<p>Soutenance : 24 septembre 2025 \u00e0 9h00<strong><br \/>\n<\/strong>Amphith\u00e9\u00e2tre de l&rsquo;IRCICA\/IRI \u2013 Campus CNRS Haute Borne (50 av. de Halley, 59658 Villeneuve d\u2019Ascq)<\/p>\n<\/div><\/section>\n<section  class='av_textblock_section av-jtefqx33-26fa44c544b10063385818eda37e027b'   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><div  class='hr av-kjh3zw-4dff888f744b728a1aca9b3a0971493a hr-default  avia-builder-el-6  avia-builder-el-no-sibling'><span class='hr-inner'><span class=\"hr-inner-style\"><\/span><\/span><\/div>\n<h5><span style=\"color: #800000;\">Summary:<\/span><\/h5>\n<p>Le diab\u00e8te pourrait concerner jusqu\u2019\u00e0 642 millions de personnes dans le monde d\u2019ici 2040. Le diab\u00e8te de type 1 (DT1) se caract\u00e9rise par une destruction progressive des cellules \u03b2 (BCM) du pancr\u00e9as par une r\u00e9action auto-immune. La BCM est alors r\u00e9duite \u00e0 10-50 % au moment du diagnostic, puis continue de d\u00e9cro\u00eetre. Dans le diab\u00e8te de type 2 (DT2), la fonction des cellules \u03b2 se d\u00e9t\u00e9riore \u00e9galement, accompagn\u00e9e d\u2019une perte de BCM estim\u00e9e entre 30 % et 50 % au fil des ann\u00e9es. Actuellement, il n\u2019existe aucun outil clinique permettant de mesurer la fonction des cellules \u03b2 ou la BCM chez les patients, ce qui limite l\u2019optimisation des prescriptions au moment du diagnostic et durant le suivi du traitement. Le d\u00e9veloppement de m\u00e9thodes d\u2019imagerie non invasives comme l\u2019IRM pour quantifier et suivre la BCM est donc essentiel. Dans ce contexte, le travail de th\u00e8se a consist\u00e9 \u00e0 rechercher l\u2019utilisation de nanoparticules organiques comme vecteurs pour le ciblage des cellules \u03b2 et le transport de Mn\u00b2\u207a ch\u00e9lat\u00e9 comme agent d\u2019imagerie potentiel. Nous avons \u00e9tudi\u00e9 deux types de nanoparticules, les nanocapsules lipidiques (LNCs) et les nanoparticules d\u2019albumine s\u00e9rique humaine. Nos r\u00e9sultats ont montr\u00e9 que les deux pouvaient \u00eatre fonctionnalis\u00e9es avec du Mn\u00b2\u207a ch\u00e9lat\u00e9. De fa\u00e7on notable, les LNCs non fonctionnalis\u00e9es ont induit une augmentation significative de la s\u00e9cr\u00e9tion d\u2019insuline in vitro (\u00eelots pancr\u00e9atiques isol\u00e9s de souris et cellules MIN6), comparable \u00e0 l\u2019effet de l\u2019exendine-4, un agoniste des r\u00e9cepteurs du GLP-1 (GLP-1RA). In vivo, les LNC ont am\u00e9lior\u00e9 la tol\u00e9rance au glucose et r\u00e9duit l\u2019hyperglyc\u00e9mie chez des souris saines et diab\u00e9tiques db\/db, en activant le r\u00e9cepteur du GLP-1 et la voie de l\u2019AMPc. Ces travaux sugg\u00e8rent que les LNC pourraient constituer une approche innovante \u00e0 double fonction : permettre le suivi de la BCM par imagerie et stimuler la s\u00e9cr\u00e9tion d\u2019insuline, offrant ainsi un nouvel outil pour la prise en charge int\u00e9gr\u00e9e du DT2 et du DT1 poss\u00e9dant des cellules \u03b2-r\u00e9siduelles.<\/p>\n<p><strong>Keywords:<\/strong> Nanocapsules Lipidiques- Nanoparticules d\u2019Albumine humaines \u2013 Cellules b\u00e9ta pancr\u00e9atiques-Imagerie &#8211; Diab\u00e8te<\/p>\n<h5><span style=\"color: #800000;\">Abstract:<\/span><\/h5>\n<p>Diabetes is projected to affect up to 642 million people worldwide by 2040. Type 1 diabetes (T1D) is characterized by the progressive destruction of pancreatic \u03b2-cells (\u03b2-cell mass, BCM) due to an autoimmune response. At diagnosis, BCM is reduced to 10-50% of normal levels and continues to decline over time. In type 2 diabetes (T2D), \u03b2-cell function progressively deteriorates, accompanied by an estimated 30-50% reduction in BCM over the years. Currently, no clinical tools are available to directly assess \u03b2-cell function or BCM in patients, which limits the optimization of treatment prescriptions at diagnosis and during follow-up. The development of non-invasive imaging methods, such as MRI to quantify and monitor BCM is therefore an urgent medical need. In this context, this thesis focused on studying the use of organic nanoparticles as carriers for \u03b2-cell targeting and the delivery of chelated Mn\u00b2\u207a as a potential imaging agent. We specifically investigated two types of nanoparticles, lipid nanocapsules (LNCs) and human serum albumin (HSA) nanoparticles. Our results demonstrated that both types could be functionalized with chelated Mn\u00b2\u207a. Notably, non-functionalized LNCs significantly enhanced insulin secretion in vitro (in isolated mouse pancreatic islets and MIN6 cells), an effect comparable to that of exendin-4, a glucagon-like peptide-1 receptor agonist (GLP-1RA). In vivo, LNCs improved glucose tolerance and reduced hyperglycemia in both healthy and db\/db diabetic mice by activating the GLP-1 receptor and the cAMP signaling pathway. These findings suggest that LNCs may represent an innovative dual-function strategy, enabling both BCM imaging and insulin secretion stimulation, thus offering a promising tool for the integrated management of T2D and T1D with residual b-cells.<\/p>\n<p><strong>Keywords:<\/strong> Lipid nanocapsules- Pancreatic \u03b2-cells- Human serum albumin- Imaging &#8211; Diabetes<\/p>\n<\/div><\/section>","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":71083,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3,199],"tags":[],"class_list":["post-75218","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-a-la-une","category-these"],"_links":{"self":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts\/75218","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/comments?post=75218"}],"version-history":[{"count":3,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts\/75218\/revisions"}],"predecessor-version":[{"id":75222,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts\/75218\/revisions\/75222"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/media\/71083"}],"wp:attachment":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/media?parent=75218"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/categories?post=75218"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/tags?post=75218"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}