{"id":62698,"date":"2024-02-29T10:53:53","date_gmt":"2024-02-29T08:53:53","guid":{"rendered":"https:\/\/www.iemn.fr\/?p=62698"},"modified":"2024-02-29T10:53:53","modified_gmt":"2024-02-29T08:53:53","slug":"these-de-hoon-i-faire-progresser-ladministration-de-medicaments-dans-la-cornee-comprendre-la-penetration-corneenne-et-le-role-des-nanoparticules-et-des-molecules-derivees-de-lanticorps","status":"publish","type":"post","link":"https:\/\/www.iemn.fr\/en\/these-2023\/these-de-hoon-i-faire-progresser-ladministration-de-medicaments-dans-la-cornee-comprendre-la-penetration-corneenne-et-le-role-des-nanoparticules-et-des-molecules-derivees-de-lanticorps.html","title":{"rendered":"THESIS: DE HOON I. - Advancing corneal drug delivery: understanding corneal penetration and the role of nanoparticles and antibody-derived molecules"},"content":{"rendered":"<div id='layer_slider_1'  class='avia-layerslider main_color avia-shadow  avia-builder-el-0  el_before_av_heading  avia-builder-el-first  container_wrap sidebar_right'  style='height: 261px;'  ><div id=\"layerslider_58_1i2ogcp29h2re\" data-ls-slug=\"homepageslider\" class=\"ls-wp-container fitvidsignore ls-selectable\" style=\"width:1140px;height:260px;margin:0 auto;margin-bottom: 0px;\"><div class=\"ls-slide\" data-ls=\"duration:6000;transition2d:5;\"><img loading=\"lazy\" decoding=\"async\" width=\"2600\" height=\"270\" src=\"https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1.jpg\" class=\"ls-bg\" alt=\"\" srcset=\"https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1.jpg 2600w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1-300x31.jpg 300w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1-768x80.jpg 768w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1-1030x107.jpg 1030w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1-1500x156.jpg 1500w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1-705x73.jpg 705w\" sizes=\"auto, (max-width: 2600px) 100vw, 2600px\" \/><ls-layer style=\"font-size:14px;text-align:left;font-style:normal;text-decoration:none;text-transform:none;font-weight:700;letter-spacing:0px;border-style:solid;border-color:#000;background-position:0% 0%;background-repeat:no-repeat;width:180px;height:30px;left:0px;top:231px;line-height:32px;color:#ffffff;border-radius:6px 6px 6px 6px;padding-left:50px;background-color:rgba(0, 0, 0, 0.57);\" class=\"ls-l ls-ib-icon ls-text-layer\" data-ls=\"minfontsize:0;minmobilefontsize:0;\"><i class=\"fa fa-quote-right\" style=\"color:#ffffff;margin-right:0.8em;font-size:1em;transform:translateY( -0.125em );\"><\/i>ACTUALITES<\/ls-layer><\/div><\/div><\/div><div id='after_layer_slider_1'  class='main_color av_default_container_wrap container_wrap sidebar_right'  ><div class='container av-section-cont-open' ><div class='template-page content  av-content-small alpha units'><div class='post-entry post-entry-type-page post-entry-62698'><div class='entry-content-wrapper clearfix'>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-lt6zmi02-5a07ae278fa08d772c01ef27e6172a51\">\n#top .av-special-heading.av-lt6zmi02-5a07ae278fa08d772c01ef27e6172a51{\nmargin:0 0 10px 0;\npadding-bottom:4px;\n}\nbody .av-special-heading.av-lt6zmi02-5a07ae278fa08d772c01ef27e6172a51 .av-special-heading-tag .heading-char{\nfont-size:25px;\n}\n.av-special-heading.av-lt6zmi02-5a07ae278fa08d772c01ef27e6172a51 .av-subheading{\nfont-size:15px;\n}\n<\/style>\n<div  class='av-special-heading av-lt6zmi02-5a07ae278fa08d772c01ef27e6172a51 av-special-heading-h2  avia-builder-el-1  el_after_av_layerslider  el_before_av_hr  avia-builder-el-first'><h2 class='av-special-heading-tag'  itemprop=\"headline\"  >THESE: DE HOON I. &#8211; Faire progresser l\u2018administration de m\u00e9dicaments dans la corn\u00e9e: comprendre la p\u00e9n\u00e9tration corn\u00e9enne et le r\u00f4le des nanoparticules et des mol\u00e9cules d\u00e9riv\u00e9es de l\u2019anticorps<\/h2><div class=\"special-heading-border\"><div class=\"special-heading-inner-border\"><\/div><\/div><\/div>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-18u73nj-dad6a947580930e400fc42ba200e80f1\">\n#top .hr.av-18u73nj-dad6a947580930e400fc42ba200e80f1{\nmargin-top:5px;\nmargin-bottom:5px;\n}\n.hr.av-18u73nj-dad6a947580930e400fc42ba200e80f1 .hr-inner{\nwidth:100%;\n}\n<\/style>\n<div  class='hr av-18u73nj-dad6a947580930e400fc42ba200e80f1 hr-custom  avia-builder-el-2  el_after_av_heading  el_before_av_textblock  hr-left hr-icon-no'><span class='hr-inner inner-border-av-border-thin'><span class=\"hr-inner-style\"><\/span><\/span><\/div>\n<section  class='av_textblock_section av-jriy64i8-2f4600354c0449b610997916bbd9b6bc'   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" >\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-13ewzjw-68e036126b913e5028f77311dc66b825\">\n.av_font_icon.av-13ewzjw-68e036126b913e5028f77311dc66b825{\ncolor:#bfbfbf;\nborder-color:#bfbfbf;\n}\n.av_font_icon.av-13ewzjw-68e036126b913e5028f77311dc66b825 .av-icon-char{\nfont-size:60px;\nline-height:60px;\n}\n<\/style>\n<span  class='av_font_icon av-13ewzjw-68e036126b913e5028f77311dc66b825 avia_animate_when_visible av-icon-style- avia-icon-pos-left avia-icon-animate'><span class='av-icon-char' aria-hidden='true' data-av_icon='\ue8c9' data-av_iconfont='entypo-fontello' ><\/span><\/span>\n<p><strong><br \/>\n<\/strong><u>DE HOON I.<\/u><br \/>\nTh\u00e8se de doctorat en Micro-nanosyst\u00e8mes et capteurs, Universit\u00e9 de Lille, ENGSYS Sciences de l\u2019ing\u00e9nierie et des syst\u00e8mes, 6 octobre 2023<\/p>\n<p><strong><br \/>\n<\/strong><\/p>\n<\/div><\/section>\n<section  class='av_textblock_section av-jtefqx33-628129dba2299b2ecd65ebfc92eac29d'   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><div  class='hr av-kjh3zw-4dff888f744b728a1aca9b3a0971493a hr-default  avia-builder-el-6  avia-builder-el-no-sibling'><span class='hr-inner'><span class=\"hr-inner-style\"><\/span><\/span><\/div>\n<h5>Summary:<\/h5>\n<p>Les maladies, les infections, les blessures et plusieurs dystrophies peuvent affecter la corn\u00e9e et conduire \u00e0 la c\u00e9cit\u00e9 corn\u00e9enne, qui est actuellement la quatri\u00e8me cause de c\u00e9cit\u00e9. Dans de nombreux cas, la transplantation de corn\u00e9e est la seule option de traitement qui reste aux patients pour recouvrer la vue, car aucun traitement pharmacologique permanent n&rsquo;est actuellement disponible. Cependant, la p\u00e9nurie de donneurs de corn\u00e9e dans le monde limite le nombre de patients pouvant b\u00e9n\u00e9ficier d&rsquo;une transplantation D&rsquo;autres options th\u00e9rapeutiques sont n\u00e9cessaires, mais la structure complexe de la corn\u00e9e, qui comporte de nombreuses barri\u00e8res physiologiques et anatomiques, pose des probl\u00e8mes importants pour l&rsquo;administration de m\u00e9dicaments dans l&rsquo;?il. Dans cette th\u00e8se, diff\u00e9rentes approches pour surmonter ces limitations ont \u00e9t\u00e9 con\u00e7ues et test\u00e9es en utilisant des yeux entiers de bovins excis\u00e9s comme mod\u00e8le d&rsquo;?il ex-vivo. Les quantum dots de carbone (CQDs) ont \u00e9t\u00e9 le point focal car ces nanostructures ont d\u00e9montr\u00e9 un grand potentiel pour la nanom\u00e9decine oculaire. Il a \u00e9t\u00e9 constat\u00e9 que les CQDs charg\u00e9s n\u00e9gativement ont une capacit\u00e9 de p\u00e9n\u00e9tration limit\u00e9e dans la corn\u00e9e, alors que les CQDs charg\u00e9s positivement et d\u00e9riv\u00e9s du chlorhydrate de glucosamine et de la spermidine (CQD-S) peuvent p\u00e9n\u00e9trer l&rsquo;ensemble de l&rsquo;\u00e9pith\u00e9lium corn\u00e9en jusqu&rsquo;\u00e0 l&rsquo;endoth\u00e9lium. Les propri\u00e9t\u00e9s d&rsquo;am\u00e9lioration de la p\u00e9n\u00e9tration des CQD-S ont \u00e9t\u00e9 notamment soulign\u00e9es en utilisant le dextran (150 kDa) comme mod\u00e8le. Les CQDs charg\u00e9s positivement \u00e9taient en outre d&rsquo;excellents nanostructures pour interf\u00e9rer avec l&rsquo;agr\u00e9gation du collag\u00e8ne de type I sous l\u2019illumination laser puls\u00e9e \u00e0 faible \u00e9nergie. Cette approche a permis la d\u00e9sint\u00e9gration des agr\u00e9gats de collag\u00e8ne et d&rsquo;opacit\u00e9s vitr\u00e9ennes humaines. Finalement, une \u00e9tude syst\u00e9matique des effets de la taille des immunoglobulines a d\u00e9montr\u00e9 la faisabilit\u00e9 de l&rsquo;utilisation de mol\u00e9cules d\u00e9riv\u00e9es des immunoglobulines en tant que th\u00e9rapies potentielles pour gu\u00e9rir les maladies de la corn\u00e9e et la c\u00e9cit\u00e9. Les connaissances acquises gr\u00e2ce \u00e0 ce travail ont des implications pour le d\u00e9veloppement de syst\u00e8mes innovants d&rsquo;administration de m\u00e9dicaments qui peuvent p\u00e9n\u00e9trer efficacement la corn\u00e9e et am\u00e9liorer les r\u00e9sultats des traitements pour les patients souffrant de maladies et de troubles de la corn\u00e9e.<\/p>\n<h5>Abstract:<\/h5>\n<p>Disease, infection, injury and a number of dystrophies can affect the cornea and lead to corneal blindness, which is currently the fourth leading cause of blindness. In many cases, corneal transplantation is the only treatment option left for patients to regain their sight, as no permanent pharmacological treatment is currently available. Other treatment options are needed, but the complex structure of the cornea, with its many physiological and anatomical barriers, poses significant problems for the delivery of drugs to the eye. In this thesis, different approaches to overcoming these limitations were designed and tested using whole excised bovine eyes as an ex-vivo eye model. Carbon quantum dots (CQDs) were the focal point as these nanostructures have shown great potential for ocular nanomedicine. It was found that negatively charged CQDs have a limited ability to penetrate the cornea, whereas positively charged CQDs derived from glucosamine hydrochloride and spermidine (CQD-S) can penetrate the entire corneal epithelium down to the endothelium. The penetration-enhancing properties of CQD-S were highlighted using dextran (150 kDa) as a model. The positively charged CQDs were also excellent nanostructures for interfering with type I collagen aggregation under low-energy pulsed laser illumination. This approach enabled the disintegration of collagen aggregates and human vitreous opacities. Finally, a systematic study of the effects of immunoglobulin size demonstrated the feasibility of using immunoglobulin-derived molecules as potential therapies for curing corneal disease and blindness. The knowledge gained from this work has implications for the development of innovative drug delivery systems that can effectively penetrate the cornea and improve treatment outcomes for patients suffering from corneal diseases and disorders.<\/p>\n<\/div><\/section>","protected":false},"excerpt":{"rendered":"","protected":false},"author":20,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[318],"tags":[],"class_list":["post-62698","post","type-post","status-publish","format-standard","hentry","category-these-2023"],"_links":{"self":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts\/62698","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/users\/20"}],"replies":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/comments?post=62698"}],"version-history":[{"count":0,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts\/62698\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/media?parent=62698"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/categories?post=62698"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/tags?post=62698"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}