{"id":56747,"date":"2023-03-15T12:59:52","date_gmt":"2023-03-15T10:59:52","guid":{"rendered":"https:\/\/www.iemn.fr\/?p=56747"},"modified":"2023-04-07T15:35:28","modified_gmt":"2023-04-07T13:35:28","slug":"dor-et-de-lumiere-un-melange-detonant-pour-la-transfection-cellulaire","status":"publish","type":"post","link":"https:\/\/www.iemn.fr\/en\/newsletter\/dor-et-de-lumiere-un-melange-detonant-pour-la-transfection-cellulaire.html","title":{"rendered":"Gold and Light: an explosive mix for cell transfection"},"content":{"rendered":"<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-lf3pcb-3aed36b54fdffe8c3cb178e491787aee\">\n.flex_column.av-lf3pcb-3aed36b54fdffe8c3cb178e491787aee{\nborder-radius:0px 0px 0px 0px;\npadding:0px 0px 0px 0px;\n}\n<\/style>\n<div  class='flex_column av-lf3pcb-3aed36b54fdffe8c3cb178e491787aee av_one_full  avia-builder-el-0  avia-builder-el-no-sibling  first flex_column_div av-zero-column-padding'     ><style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-lf9kh6qk-dcc830de5907e571c1a33b68bb3cd135\">\n#top .av_textblock_section.av-lf9kh6qk-dcc830de5907e571c1a33b68bb3cd135 .avia_textblock{\nfont-size:15px;\n}\n<\/style>\n<section  class='av_textblock_section av-lf9kh6qk-dcc830de5907e571c1a33b68bb3cd135'   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><h1 class=\"\" style=\"text-align: center;\">Of Gold and Light :<br \/>\na detonating mixture for cell transfection<\/h1>\n<div class=\"row\">\n<div class=\"col-md-8 col-lg-8\">\n<div class=\"teaser-text\"><strong><span style=\"color: #800000;\">As part of the ERC Nanobubble programme, publication of an article resulting from an interdisciplinary collaboration between UGhent and U-Lille (Canther, IEMN, PhLAM) in the Journal of Nanobiotechnology.<\/span><\/strong><\/div>\n<div class=\"news-text-wrap\">\n<blockquote>\n<p><a href=\"https:\/\/www.iemn.fr\/wp-content\/uploads\/2023\/03\/csm_article-ERC_Nanobubble_0444d49c6a.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignright size-medium wp-image-56748\" src=\"https:\/\/www.iemn.fr\/wp-content\/uploads\/2023\/03\/csm_article-ERC_Nanobubble_0444d49c6a-291x300.jpg\" alt=\"\" width=\"291\" height=\"300\" srcset=\"https:\/\/www.iemn.fr\/wp-content\/uploads\/2023\/03\/csm_article-ERC_Nanobubble_0444d49c6a-291x300.jpg 291w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2023\/03\/csm_article-ERC_Nanobubble_0444d49c6a-12x12.jpg 12w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2023\/03\/csm_article-ERC_Nanobubble_0444d49c6a-36x36.jpg 36w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2023\/03\/csm_article-ERC_Nanobubble_0444d49c6a.jpg 450w\" sizes=\"auto, (max-width: 291px) 100vw, 291px\" \/><\/a>Intracellular administration of molecules is a key stage in many diagnostic and therapeutic procedures. Several 'transfection' techniques exist (viral or non-viral vectors, physical methods) to circumvent the various obstacles to the controlled introduction of drugs, while optimising key parameters such as efficacy, viability and treatment rate.<\/p>\n<p><strong>In the register of physical methods, photoporation with gold nanoparticles has shown to be a promising approach to permeabilize cell membranes through the generation of surface plasmon resonance (SPR)-induced vapor nanobubbles with a laser, allowing the entry of external molecules or cargoes inside the cells.<\/strong><\/p>\n<p>As part of the<strong>ERC Nanobubble<\/strong> (K. Braeckmans, <a href=\"https:\/\/www.ugent.be\/en\" target=\"_blank\" rel=\"noopener noreferrer\">UGhent<\/a>) and interdisciplinary collaboration between Canther laboratories, <a href=\"https:\/\/www.iemn.fr\/en\/\" rel=\"noopener noreferrer\">IEMN<\/a> and <a href=\"https:\/\/phlam.univ-lille.fr\/\" target=\"_blank\" rel=\"noopener\">PhLAM<\/a>A new optofluidic method enables gold nanoparticles to be held in close proximity to cells for photoporation, while avoiding direct contact with the cells by taking advantage of hydrodynamic focusing in a multi-flow microfluidic device.<\/p>\n<p class=\"translation-block\">Cells are photoporinated with an efficiency of \u223c 70% without significant reduction in cell viability at a rate ranging from 10<sup>3<\/sup> to 10<sup>4<\/sup> cells per minute. This optofluidic approach offers prospects for transferring photoporation to clinical use for the production of genetically engineered therapeutic cells.<\/p>\n<p class=\"translation-block\">This work was just published recently (February 2023) in the Journal of Nanobiotechnology (5-year IF 11.5): <a href=\"https:\/\/rdcu.be\/c5dSY\" target=\"_blank\" rel=\"noopener noreferrer\">https:\/\/rdcu.be\/c5dSY<\/a><\/p>\n<\/blockquote>\n<p><em>M. Layachi, A. Treizebr\u00e9, L. Hay, D. Gilbert, J. Pesez, Q. D\u2019Acremont, K. Braeckmans, Q. Thommen and E. Courtade, Novel opto\u2011fluidic drug delivery system for efficient cellular transfection, J. Nanobiotechnology <strong>21<\/strong>, 43 (2023)<a href=\"https:\/\/www.iemn.fr\/wp-content\/uploads\/2023\/03\/csm_ERC_Nanobubble_2de945418a.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-56749 aligncenter\" src=\"https:\/\/www.iemn.fr\/wp-content\/uploads\/2023\/03\/csm_ERC_Nanobubble_2de945418a-295x300.jpg\" alt=\"\" width=\"246\" height=\"250\" srcset=\"https:\/\/www.iemn.fr\/wp-content\/uploads\/2023\/03\/csm_ERC_Nanobubble_2de945418a-295x300.jpg 295w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2023\/03\/csm_ERC_Nanobubble_2de945418a-80x80.jpg 80w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2023\/03\/csm_ERC_Nanobubble_2de945418a-12x12.jpg 12w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2023\/03\/csm_ERC_Nanobubble_2de945418a-36x36.jpg 36w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2023\/03\/csm_ERC_Nanobubble_2de945418a.jpg 450w\" sizes=\"auto, (max-width: 246px) 100vw, 246px\" \/><\/a><\/em><\/p>\n<\/div>\n<p><strong>Contact PhLAM :\u00a0<\/strong><a href=\"mailto:emmanuel.courtade@univ-lille.fr\">emmanuel.courtade@univ-lille<span class=\"point\">.<\/span>fr<\/a><\/p>\n<\/div>\n<div class=\"col-md-8 col-lg-8\"><\/div>\n<div class=\"col-md-4 col-lg-4\">\n<div class=\"news-img-wrap\">\n<div class=\"outer\">\n<div class=\"mediaelement mediaelement-image\"><\/div>\n<\/div>\n<div class=\"outer\">\n<div class=\"mediaelement mediaelement-image\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<hr \/>\n<div class=\"news-related-wrap\">\n<div><\/div>\n<\/div>\n<div class=\"news-backlink-wrap align-right\"><\/div>\n<div class=\"labo-wrapper bigWrap site-23115\">\n<footer id=\"footerLabo\" class=\"labo-footer\">\n<div class=\"container\">\n<div class=\"row pt-2 pt-2\">\n<div class=\"col-12 col-md-3 mentions\">\n<div class=\"copyright\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/footer>\n<\/div>\n<div id=\"tarteaucitronRoot\" class=\"tarteaucitronBeforeVisible\" lang=\"fr\" role=\"region\" data-nosnippet=\"true\" aria-labelledby=\"tac_title\">\n<div id=\"tarteaucitronAlertBig\" class=\"tarteaucitronAlertBigBottom\" tabindex=\"-1\"><\/div>\n<\/div>\n<\/div><\/section><\/div>","protected":false},"excerpt":{"rendered":"","protected":false},"author":20,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[297],"tags":[],"class_list":["post-56747","post","type-post","status-publish","format-standard","hentry","category-newsletter"],"_links":{"self":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts\/56747","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/users\/20"}],"replies":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/comments?post=56747"}],"version-history":[{"count":0,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts\/56747\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/media?parent=56747"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/categories?post=56747"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/tags?post=56747"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}