{"id":55559,"date":"2022-12-01T15:37:10","date_gmt":"2022-12-01T13:37:10","guid":{"rendered":"https:\/\/www.iemn.fr\/?p=55559"},"modified":"2022-12-01T15:37:10","modified_gmt":"2022-12-01T13:37:10","slug":"these-q-rezard-identification-en-haut-debit-de-cellules-cancereuses-circulantes-par-leur-signature-biophysique-rezard-q","status":"publish","type":"post","link":"https:\/\/www.iemn.fr\/en\/theses-2022\/these-q-rezard-identification-en-haut-debit-de-cellules-cancereuses-circulantes-par-leur-signature-biophysique-rezard-q.html","title":{"rendered":"THESE : Q. REZARD \u2013 Identification en haut d\u00e9bit de cellules canc\u00e9reuses circulantes par leur signature biophysique  REZARD Q."},"content":{"rendered":"<div id='layer_slider_1'  class='avia-layerslider main_color avia-shadow  avia-builder-el-0  el_before_av_heading  avia-builder-el-first  container_wrap sidebar_right'  style='height: 261px;'  ><div id=\"layerslider_58_i198bzkfvsk7\" data-ls-slug=\"homepageslider\" class=\"ls-wp-container fitvidsignore ls-selectable\" style=\"width:1140px;height:260px;margin:0 auto;margin-bottom: 0px;\"><div class=\"ls-slide\" data-ls=\"duration:6000;transition2d:5;\"><img loading=\"lazy\" decoding=\"async\" width=\"2600\" height=\"270\" src=\"https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1.jpg\" class=\"ls-bg\" alt=\"\" srcset=\"https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1.jpg 2600w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1-300x31.jpg 300w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1-768x80.jpg 768w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1-1030x107.jpg 1030w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1-1500x156.jpg 1500w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1-705x73.jpg 705w\" sizes=\"auto, (max-width: 2600px) 100vw, 2600px\" \/><ls-layer style=\"font-size:14px;text-align:left;font-style:normal;text-decoration:none;text-transform:none;font-weight:700;letter-spacing:0px;border-style:solid;border-color:#000;background-position:0% 0%;background-repeat:no-repeat;width:180px;height:30px;left:0px;top:231px;line-height:32px;color:#ffffff;border-radius:6px 6px 6px 6px;padding-left:50px;background-color:rgba(0, 0, 0, 0.57);\" class=\"ls-l ls-ib-icon ls-text-layer\" data-ls=\"minfontsize:0;minmobilefontsize:0;\"><i class=\"fa fa-quote-right\" style=\"color:#ffffff;margin-right:0.8em;font-size:1em;transform:translateY( -0.125em );\"><\/i>ACTUALITES<\/ls-layer><\/div><\/div><\/div><div id='after_layer_slider_1'  class='main_color av_default_container_wrap container_wrap sidebar_right'  ><div class='container av-section-cont-open' ><div class='template-page content  av-content-small alpha units'><div class='post-entry post-entry-type-page post-entry-55559'><div class='entry-content-wrapper clearfix'>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-lb54cik9-228c854a644c6734ebc21ff6ee08af87\">\n#top .av-special-heading.av-lb54cik9-228c854a644c6734ebc21ff6ee08af87{\nmargin:0 0 10px 0;\npadding-bottom:4px;\n}\nbody .av-special-heading.av-lb54cik9-228c854a644c6734ebc21ff6ee08af87 .av-special-heading-tag .heading-char{\nfont-size:25px;\n}\n.av-special-heading.av-lb54cik9-228c854a644c6734ebc21ff6ee08af87 .av-subheading{\nfont-size:15px;\n}\n<\/style>\n<div  class='av-special-heading av-lb54cik9-228c854a644c6734ebc21ff6ee08af87 av-special-heading-h2  avia-builder-el-1  el_after_av_layerslider  el_before_av_hr  avia-builder-el-first'><h2 class='av-special-heading-tag'  itemprop=\"headline\"  >THESE : Q. REZARD &#8211; Identification en haut d\u00e9bit de cellules canc\u00e9reuses circulantes par leur signature biophysique REZARD Q. <\/h2><div class=\"special-heading-border\"><div class=\"special-heading-inner-border\"><\/div><\/div><\/div>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-18u73nj-dad6a947580930e400fc42ba200e80f1\">\n#top .hr.av-18u73nj-dad6a947580930e400fc42ba200e80f1{\nmargin-top:5px;\nmargin-bottom:5px;\n}\n.hr.av-18u73nj-dad6a947580930e400fc42ba200e80f1 .hr-inner{\nwidth:100%;\n}\n<\/style>\n<div  class='hr av-18u73nj-dad6a947580930e400fc42ba200e80f1 hr-custom  avia-builder-el-2  el_after_av_heading  el_before_av_textblock  hr-left hr-icon-no'><span class='hr-inner inner-border-av-border-thin'><span class=\"hr-inner-style\"><\/span><\/span><\/div>\n<section  class='av_textblock_section av-jriy64i8-2f4600354c0449b610997916bbd9b6bc'   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" >\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-13ewzjw-68e036126b913e5028f77311dc66b825\">\n.av_font_icon.av-13ewzjw-68e036126b913e5028f77311dc66b825{\ncolor:#bfbfbf;\nborder-color:#bfbfbf;\n}\n.av_font_icon.av-13ewzjw-68e036126b913e5028f77311dc66b825 .av-icon-char{\nfont-size:60px;\nline-height:60px;\n}\n<\/style>\n<span  class='av_font_icon av-13ewzjw-68e036126b913e5028f77311dc66b825 avia_animate_when_visible av-icon-style- avia-icon-pos-left avia-icon-animate'><span class='av-icon-char' aria-hidden='true' data-av_icon='\ue8c9' data-av_iconfont='entypo-fontello' ><\/span><\/span>\n<p><strong>Q. REZARDN<\/strong><\/p>\n<p>Soutenance : <strong>20 mai 2022<br \/>\n<\/strong>Th\u00e8se de doctorat en Electronique, micro\u00e9lectronique, nano\u00e9lectronique et micro-ondes, Universit\u00e9 de Lille, ENGSYS Sciences de l\u2019ing\u00e9nierie et des syst\u00e8mes,<\/p>\n<\/div><\/section>\n<section  class='av_textblock_section av-jtefqx33-628129dba2299b2ecd65ebfc92eac29d'   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><div  class='hr av-kjh3zw-4dff888f744b728a1aca9b3a0971493a hr-default  avia-builder-el-6  avia-builder-el-no-sibling'><span class='hr-inner'><span class=\"hr-inner-style\"><\/span><\/span><\/div>\n<h5>Summary:<\/h5>\n<p>Une tumeur lib\u00e8re divers marqueurs dans son environnement. Parmi eux, les cellules tumorales circulantes (CTC) se propagent dans les vaisseaux sanguins cr\u00e9ant ensuite des m\u00e9tastases. Les avantages de la biopsie liquide ont mis en avant la d\u00e9tection des CTCs comme un potentiel outil de diagnostic. Cependant, leur he?te?roge?ne?ite? et leur faible concentration ne?cessitent la capacite? d\u2019analyser inde?pendamment chaque cellule d\u2019un e?chantillon avec une me?thode fiable et a? haut de?bit. Pour pouvoir e?tre exploite?e dans un contexte me?dical, cette me?thode doit e?tre a? la fois pratique dans sa mise en place et fiable en termes de gestion des donne?es. La seule me?thode approuve?e cible les marqueurs de surface des cellules. Cependant, certaines cellules cance?reuses perdent ces marqueurs lors de leur transition e?pithe?liale \/me?senchymateuse. Des me?thodes innovantes ciblant de nouveaux biomarqueurs sont donc indispensables. Les marqueurs biophysiques apparaissent pertinents car directement relie?s a? l\u2019e?volution des CTCs lors de leur migration. La microfluidique a permis l\u2019introduction d\u2019approches originales d\u2019analyse a? haut de?bit. Cependant, un compromis existe entre le temps ne?cessaire a? l\u2019analyse d\u2019une cellule et la richesse des informations obtenues. La combinaison des caracte?risations e?lectriques et me?caniques parai?t e?tre la meilleure approche afin d\u2019atteindre un rendement de mesure pertinent et exploitable. La cytome?trie d\u2019impe?dance nous est apparue comme une technologie de base tre?s pertinente afin d\u2019y associer la caracte?risation me?canique. Cependant, les me?thodes de caracte?risations me?caniques les plus utilise?es en microfluidique, telle que la cytome?trie de de?formabilite?, pre?sente de fortes limitations expe?rimentales soit lie?es a? l\u2019obstruction de canaux ou au besoin de traitement massif de donne?es d\u2019image associe?e au tre?s haut de?bit. Dans ce contexte, ce travail vise une me?thode fiable, pratique et a? haut de?bit afin de caracte?riser les ligne?es cance?reuses en fonction de leur signature biophysique. Un dispositif combinant MEMS et microfluidique est propos\u00e9 ou? des paires d\u2019e?lectrodes en silicium inte?gre?es dans les parois du canal effectuent la cytome?trie d\u2019impe?dance tandis que les caracte?ristiques me?caniques sont obtenues par la mesure de la compression physique des cellules dans un entrefer e?troit pourvu d\u2019un capteur de de?placement.<\/p>\n<h5>Abstract:<\/h5>\n<p>TA tumor releases various markers into its environment. Among them, circulating tumor cells (CTCs) spread in blood vessels and metastasize. The advantages of liquid biopsy have highlighted the detection of CTCs as a potential diagnostic tool. However, their heterogeneity and low concentration require the ability to analyze each cell in a sample independently with a reliable and high throughput method. To be used in a medical context, this method must be both practical in its implementation and reliable in terms of data management. The only approved method targets cell surface markers. However, some cancer cells lose these markers during their epithelial\/mesenchymal transition. Innovative methods targeting new biomarkers are therefore essential. Biophysical markers appear relevant because they are directly related to the evolution of CTCs during their migration. Microfluidics has allowed the introduction of original high throughput analysis approaches. However, there is a trade-off between the time needed to analyze a cell and the richness of the information obtained. The combination of electrical and mechanical characterizations seems to be the best approach to reach a relevant and exploitable measurement yield. Imaging cytometry appeared to be a very relevant basic technology to combine with mechanical characterization. However, the most commonly used methods of mechanical characterization in microfluidics, such as deformability cytometry, present strong experimental limitations either related to channel obstruction or to the need for massive image data processing associated with very high throughput. In this context, this work aims at a reliable, practical and high-definition method to characterize the faulty lines according to their biophysical signature. A device combining MEMS and microfluidics is proposed where pairs of silicon electrodes integrated in the walls of the channel perform the imaging cytometry while the mechanical characteristics are obtained by measuring the physical compression of the cells in a narrow air gap equipped with a deposition sensor.<\/p>\n<\/div><\/section>","protected":false},"excerpt":{"rendered":"","protected":false},"author":20,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[316],"tags":[],"class_list":["post-55559","post","type-post","status-publish","format-standard","hentry","category-theses-2022"],"_links":{"self":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts\/55559","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/users\/20"}],"replies":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/comments?post=55559"}],"version-history":[{"count":0,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts\/55559\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/media?parent=55559"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/categories?post=55559"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/tags?post=55559"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}