{"id":55556,"date":"2022-12-01T15:29:01","date_gmt":"2022-12-01T13:29:01","guid":{"rendered":"https:\/\/www.iemn.fr\/?p=55556"},"modified":"2022-12-01T15:29:01","modified_gmt":"2022-12-01T13:29:01","slug":"these-m-layachi-developpement-dun-dispositif-microfluidique-pour-la-photoporation-de-cellules-biologiques-a-hauts-debits","status":"publish","type":"post","link":"https:\/\/www.iemn.fr\/en\/these\/these-2021\/these-m-layachi-developpement-dun-dispositif-microfluidique-pour-la-photoporation-de-cellules-biologiques-a-hauts-debits.html","title":{"rendered":"THESE : M. LAYACHI &#8211; D\u00e9veloppement d&rsquo;un dispositif microfluidique pour la photoporation de cellules biologiques \u00e0 hauts d\u00e9bits"},"content":{"rendered":"<div id='layer_slider_1'  class='avia-layerslider main_color avia-shadow  avia-builder-el-0  el_before_av_heading  avia-builder-el-first  container_wrap sidebar_right'  style='height: 261px;'  ><div id=\"layerslider_58_o5xb5pwn4pmw\" data-ls-slug=\"homepageslider\" class=\"ls-wp-container fitvidsignore ls-selectable\" style=\"width:1140px;height:260px;margin:0 auto;margin-bottom: 0px;\"><div class=\"ls-slide\" data-ls=\"duration:6000;transition2d:5;\"><img loading=\"lazy\" decoding=\"async\" width=\"2600\" height=\"270\" src=\"https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1.jpg\" class=\"ls-bg\" alt=\"\" srcset=\"https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1.jpg 2600w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1-300x31.jpg 300w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1-768x80.jpg 768w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1-1030x107.jpg 1030w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1-1500x156.jpg 1500w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2019\/01\/sliders_news1-705x73.jpg 705w\" sizes=\"auto, (max-width: 2600px) 100vw, 2600px\" \/><ls-layer style=\"font-size:14px;text-align:left;font-style:normal;text-decoration:none;text-transform:none;font-weight:700;letter-spacing:0px;border-style:solid;border-color:#000;background-position:0% 0%;background-repeat:no-repeat;width:180px;height:30px;left:0px;top:231px;line-height:32px;color:#ffffff;border-radius:6px 6px 6px 6px;padding-left:50px;background-color:rgba(0, 0, 0, 0.57);\" class=\"ls-l ls-ib-icon ls-text-layer\" data-ls=\"minfontsize:0;minmobilefontsize:0;\"><i class=\"fa fa-quote-right\" style=\"color:#ffffff;margin-right:0.8em;font-size:1em;transform:translateY( -0.125em );\"><\/i>ACTUALITES<\/ls-layer><\/div><\/div><\/div><div id='after_layer_slider_1'  class='main_color av_default_container_wrap container_wrap sidebar_right'  ><div class='container av-section-cont-open' ><div class='template-page content  av-content-small alpha units'><div class='post-entry post-entry-type-page post-entry-55556'><div class='entry-content-wrapper clearfix'>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-lb543hng-75a580fbde0b2244f438cb73afaa7af7\">\n#top .av-special-heading.av-lb543hng-75a580fbde0b2244f438cb73afaa7af7{\nmargin:0 0 10px 0;\npadding-bottom:4px;\n}\nbody .av-special-heading.av-lb543hng-75a580fbde0b2244f438cb73afaa7af7 .av-special-heading-tag .heading-char{\nfont-size:25px;\n}\n.av-special-heading.av-lb543hng-75a580fbde0b2244f438cb73afaa7af7 .av-subheading{\nfont-size:15px;\n}\n<\/style>\n<div  class='av-special-heading av-lb543hng-75a580fbde0b2244f438cb73afaa7af7 av-special-heading-h2  avia-builder-el-1  el_after_av_layerslider  el_before_av_hr  avia-builder-el-first'><h2 class='av-special-heading-tag'  itemprop=\"headline\"  >THESE : M. LAYACHI &#8211; D\u00e9veloppement d\u2019un dispositif microfluidique pour la photoporation de cellules biologiques \u00e0 hauts d\u00e9bits <\/h2><div class=\"special-heading-border\"><div class=\"special-heading-inner-border\"><\/div><\/div><\/div>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-18u73nj-dad6a947580930e400fc42ba200e80f1\">\n#top .hr.av-18u73nj-dad6a947580930e400fc42ba200e80f1{\nmargin-top:5px;\nmargin-bottom:5px;\n}\n.hr.av-18u73nj-dad6a947580930e400fc42ba200e80f1 .hr-inner{\nwidth:100%;\n}\n<\/style>\n<div  class='hr av-18u73nj-dad6a947580930e400fc42ba200e80f1 hr-custom  avia-builder-el-2  el_after_av_heading  el_before_av_textblock  hr-left hr-icon-no'><span class='hr-inner inner-border-av-border-thin'><span class=\"hr-inner-style\"><\/span><\/span><\/div>\n<section  class='av_textblock_section av-jriy64i8-2f4600354c0449b610997916bbd9b6bc'   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" >\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-13ewzjw-68e036126b913e5028f77311dc66b825\">\n.av_font_icon.av-13ewzjw-68e036126b913e5028f77311dc66b825{\ncolor:#bfbfbf;\nborder-color:#bfbfbf;\n}\n.av_font_icon.av-13ewzjw-68e036126b913e5028f77311dc66b825 .av-icon-char{\nfont-size:60px;\nline-height:60px;\n}\n<\/style>\n<span  class='av_font_icon av-13ewzjw-68e036126b913e5028f77311dc66b825 avia_animate_when_visible av-icon-style- avia-icon-pos-left avia-icon-animate'><span class='av-icon-char' aria-hidden='true' data-av_icon='\ue8c9' data-av_iconfont='entypo-fontello' ><\/span><\/span>\n<p><strong>M. LAYACHI<\/strong><\/p>\n<p>Soutenance : <strong>27 janvier 2021<br \/>\n<\/strong>Doctoral thesis in Micro-nanosystems and Sensors, University of Lille,<br \/>\nAssociated project: RENATECH<\/p>\n<\/div><\/section>\n<section  class='av_textblock_section av-jtefqx33-628129dba2299b2ecd65ebfc92eac29d'   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><div  class='hr av-kjh3zw-4dff888f744b728a1aca9b3a0971493a hr-default  avia-builder-el-6  avia-builder-el-no-sibling'><span class='hr-inner'><span class=\"hr-inner-style\"><\/span><\/span><\/div>\n<h5>Summary:<\/h5>\n<p>La transfection de cellules vise \u00e0 injecter du mat\u00e9riel g\u00e9n\u00e9tique dans des cellules biologiques vivantes. L&rsquo;incorporation intracellulaire de mol\u00e9cules exog\u00e8nes est un processus n\u00e9cessaire en diagnostique et une \u00e9tape clef dans la th\u00e9rapie g\u00e9nique. Ces enjeux n\u00e9cessitent des performances en terme d&rsquo;efficacit\u00e9 (cellules viables positives), de qualit\u00e9 (incorporation) et de rendement (cadence de traitement). Des recherches sur les m\u00e9canismes limitant l&rsquo;incorporation naturelle de macromol\u00e9cules (endocytose) sont men\u00e9es et des techniques sont d\u00e9velopp\u00e9es pour surmonter les barri\u00e8res cellulaires et r\u00e9aliser le transfert intracellulaire de mani\u00e8re contr\u00f4l\u00e9e. Cependant, ces syst\u00e8mes biologiques, chimiques ou physiques pr\u00e9sentent certains inconv\u00e9nients. La photoporation par interm\u00e9diaire de nanoparticules d&rsquo;or (AuNP) r\u00e9alise n\u00e9anmoins des performances int\u00e9ressantes en perm\u00e9abilisant les membranes cellulaires gr\u00e2ce \u00e0 des nano-bulles de vapeur (VNB) g\u00e9n\u00e9r\u00e9es par laser. Une nouvelle approche est pr\u00e9sent\u00e9e ici de photoporation en environnement microfluidique dans le but d&rsquo;am\u00e9liorer le rendement et de garantir la s\u00e9paration entre les cellules et les AuNP afin d&rsquo;obtenir un \u00e9chantillon sans \u00e9l\u00e9ments cytotoxiques. Nous avons d\u00e9velopp\u00e9 un nouveau syst\u00e8me opto-fluidique int\u00e9grant des AuNP en suspension pour g\u00e9n\u00e9rer des VNB au voisinage des cellules. Cette m\u00e9thode permet un rendement et un taux de transfection \u00e9lev\u00e9s et r\u00e9duit la cytotoxicit\u00e9 de la transfection. L&rsquo;approche permet le contr\u00f4le de la distance entre cellules et AuNP. En l&rsquo;\u00e9tat, le rendement du dispositif peut atteindre 10\u00b3 \u00e0 10<sup>4<\/sup> cell. \/min . Surtout, la s\u00e9paration r\u00e9alise une meilleure viabilit\u00e9 des cellules photopor\u00e9es en comparaison avec la m\u00e9thode sans s\u00e9paration (80% contre 40%) mais r\u00e9duit le taux de transfection (30% contre 50%) : l&rsquo;augmentation de la distance am\u00e9liore la viabilit\u00e9. Outil rh\u00e9ologique ou de transfection, ce dispositif est une \u00e9tape suppl\u00e9mentaire vers la transfection efficace et bio-compatible de cellule unique, un atout majeur pour le d\u00e9veloppement clinique des nouvelles th\u00e9rapies cellulaires.<\/p>\n<h5>Abstract:<\/h5>\n<p>Cell transfection aims at injecting genetic material into living biological cells. The intracellular incorporation of exogenous molecules is a necessary process in diagnostics and a key step in gene therapy. These challenges require performance in terms of efficiency (viable positive cells), quality (incorporation) and yield (treatment rate). Research on the mechanisms limiting the natural incorporation of macromolecules (endocytosis) is being conducted and techniques are being developed to overcome cellular barriers and perform intracellular transfer in a controlled manner. However, these biological, chemical or physical systems have some drawbacks. However, gold nanoparticle (AuNP) mediated photoporation achieves interesting performances by permeabilizing cell membranes with laser generated vapour nanobubbles (VNBs). A new approach is presented here for photoporation in a microfluidic environment in order to improve the yield and to guarantee the separation between cells and AuNPs in order to obtain a sample free of cytotoxic elements. We have developed a new optofluidic system integrating AuNPs in suspension to generate VNBs in the vicinity of the cells. This method allows high yield and transfection rate and reduces transfection cytotoxicity. The approach allows control of the distance between cells and AuNPs. As it is, the device yield can reach 10\u00b3 to 104 cells\/min . Most importantly, the separation achieves a better viability of photoporinated cells compared to the method without separation (80% vs. 40%) but reduces the transfection rate (30% vs. 50%): increasing the distance improves viability. As a rheological or transfection tool, this device is a further step towards efficient and bio-compatible single cell transfection, a major asset for the clinical development of new cell therapies.<\/p>\n<\/div><\/section>","protected":false},"excerpt":{"rendered":"","protected":false},"author":20,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[317],"tags":[],"class_list":["post-55556","post","type-post","status-publish","format-standard","hentry","category-these-2021"],"_links":{"self":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts\/55556","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/users\/20"}],"replies":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/comments?post=55556"}],"version-history":[{"count":0,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts\/55556\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/media?parent=55556"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/categories?post=55556"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/tags?post=55556"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}