{"id":52858,"date":"2022-06-02T12:08:34","date_gmt":"2022-06-02T10:08:34","guid":{"rendered":"https:\/\/www.iemn.fr\/?p=52858"},"modified":"2022-06-23T11:18:45","modified_gmt":"2022-06-23T09:18:45","slug":"these-de-mohamed-taieb-bakouche-fonctionnalisation-de-surface-et-developpement-de-biocapteurs-spr-microfluidiques-pour-la-detection-du-zymoseptoria-tritici-et-de-candida-albicans","status":"publish","type":"post","link":"https:\/\/www.iemn.fr\/en\/actualites\/these-de-mohamed-taieb-bakouche-fonctionnalisation-de-surface-et-developpement-de-biocapteurs-spr-microfluidiques-pour-la-detection-du-zymoseptoria-tritici-et-de-candida-albicans.html","title":{"rendered":"Thesis by Mohamed Taieb BAKOUCHE : \u00ab\u00a0Fonctionnalisation de surface et d\u00e9veloppement de biocapteurs SPR microfluidiques pour la d\u00e9tection du Zymoseptoria tritici et de Candida albicans\u00a0\u00bb"},"content":{"rendered":"<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-av_one_full-25347b0a799d5b9f7058434b2a9c3182\">\n.flex_column.av-av_one_full-25347b0a799d5b9f7058434b2a9c3182{\nborder-radius:0px 0px 0px 0px;\npadding:0px 0px 0px 0px;\n}\n<\/style>\n<div  class='flex_column av-av_one_full-25347b0a799d5b9f7058434b2a9c3182 av_one_full  avia-builder-el-0  avia-builder-el-no-sibling  first flex_column_div av-zero-column-padding'     ><section  class='av_textblock_section av-l3wupk6s-b6a81c5d1c88581e758c80330d437a16'   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><h3 style=\"text-align: center;\">Th\u00e8se de Mohamed Taieb BAKOUCHE<br \/>\n\u00ab\u00a0Fonctionnalisation de surface et d\u00e9veloppement de biocapteurs SPR microfluidiques<br \/>\npour la d\u00e9tection du Zymoseptoria tritici et de Candida albicans\u00a0\u00bb<\/h3>\n<blockquote>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-av_font_icon-05c1c341a8e19041155b26735ecd569c\">\n.av_font_icon.av-av_font_icon-05c1c341a8e19041155b26735ecd569c .av-icon-char{\nfont-size:25px;\nline-height:25px;\n}\n<\/style>\n<span  class='av_font_icon av-av_font_icon-05c1c341a8e19041155b26735ecd569c avia_animate_when_visible av-icon-style- avia-icon-pos-left av-no-color avia-icon-animate'><span class='av-icon-char' aria-hidden='true' data-av_icon='\ue8c9' data-av_iconfont='entypo-fontello' ><\/span><\/span>\n<p>Soutenance le 10\/06\/2022 \u00e0 10h<br \/>\nAmphitheater of the IEMN - Central Laboratory - Villeneuve d'Ascq<\/p>\n<\/blockquote>\n<h4>Jury :<\/h4>\n<p>Eric FINOT, Professeur, Universit\u00e9 de Bourgogne, Laboratoire Interdisciplinaire Carnot de Bourgogne, Rapporteur<br \/>\nYoann ROUPIOZ, Directeur de Recherche CNRS, Syst\u00e8mes Mol\u00e9culaires et nanoMat\u00e9riaux pour l\u2019\u00c9nergie et la Sant\u00e9, Grenoble, Rapporteur<br \/>\nSylvain DESPREZ Examinateur Ing\u00e9nieur, Materia Nova (Mons, BE), Rapporteur<br \/>\nDeborah LANTERBECQ, Enseignant-chercheur, Haute Ecole Provinciale de Hainaut \u2013 Condorcet Laboratoire de recherche et de service en Biotechnologie et Biologie Appliqu\u00e9e (Ath, BE), Examinateur<br \/>\nBoualem SENDID, Professeur, Universit\u00e9 de Lille, Centre Hospitalier Universitaire de Lille, Institut de Microbiologie Centre de Biologie Pathologie G\u00e9n\u00e9tique (Lille), Examinateur<br \/>\nSophie MARICOT, Ma\u00eetre de Conf\u00e9rences, Universit\u00e9 de Lille, IEMN, Encadrante<br \/>\nMohamed BOUAZAOUI, Professeur, Universit\u00e9 de Lille, Laboratoire de Physique des Lasers, Atomes et Mol\u00e9cules, Co-Directeur<br \/>\nJean-Pierre VILCOT, Directeur de Recherche CNRS, IEMN, Co-Directeur<\/p>\n<h4>Summary:<\/h4>\n<p>La d\u00e9tection d\u2019\u00e9l\u00e9ments biologiques dans des milieux, qu\u2019ils soient simples ou complexes, peut \u00eatre r\u00e9alis\u00e9e par diverses m\u00e9thodes analytiques telles qu\u2019ELISA, PCR, QCM\u2026 . Parmi celles-ci, la m\u00e9thode optique qui repose sur l\u2019utilisation de biocapteurs \u00e0 base de r\u00e9sonance plasmonique de surface (SPR) est pr\u00e9sent\u00e9e comme une technique pr\u00e9sentant certains avantages: sensibilit\u00e9, analyses en temps r\u00e9el et sans marquage.<br \/>\nLes travaux de cette th\u00e8se men\u00e9s dans le cadre du projet Biosens, ont port\u00e9 sur le d\u00e9veloppement de biocapteurs SPR sans marqueur biologique pour des applications biom\u00e9dicales et agricoles. Concernant la mise en oeuvre sur le terrain, des capteurs SPR avec microfluidique int\u00e9gr\u00e9e ont \u00e9t\u00e9 d\u00e9velopp\u00e9s permettant \u00e0 terme une utilisation de terrain simplifi\u00e9e.<br \/>\nAfin de s\u2019assurer la sp\u00e9cificit\u00e9 de d\u00e9tection, ce type de capteurs n\u00e9cessite une fonctionnalisation de la surface qui est, dans notre cas, bas\u00e9e sur des anticorps ou des fragments d\u2019ADN. Deux pathog\u00e8nes diff\u00e9rentes, appartenant au r\u00e8gne fongique, \u00e0 savoir Candida Albicans et Zymoseptoria Tritici, respectivement dans les domaines m\u00e9dical et agricole, ont \u00e9t\u00e9 \u00e9tudi\u00e9es, d\u00e9tect\u00e9s et quantifi\u00e9s.<br \/>\n\u00c0 cette fin, les ligands d\u2019int\u00e9r\u00eat ont d\u2019abord \u00e9t\u00e9 immobilis\u00e9s sur la surface en or des capteurs SPR par couplage amine pour les anticorps et par liaison covalente directe thiol-or pour les monobrins d\u2019ADN. Ensuite, des \u00e9tudes d\u2019interaction biologique avec les antig\u00e8nes compl\u00e9mentaires respectifs et l\u2019ADN ont \u00e9t\u00e9 men\u00e9es en temps r\u00e9el \u00e0 l\u2019aide de deux syst\u00e8mes de lecture SPR d\u00e9velopp\u00e9s en laboratoire; le premier, fixe, bas\u00e9 sur une interrogation angulaire et le deuxi\u00e8me, portable, fonctionnant selon la configuration spectrale.<\/p>\n<h4>Abstract:<\/h4>\n<p>The detection of biological elements in complex or simple media can be achieved by various analytical methods (ELISA, PCR, QCM\u2026). Among these techniques, one can mention the optical method based on the use of surface plasmon resonance (SPR) biosensors.<br \/>\nThe work of this thesis, which is part of the Biosens project, focused on the development of biological label-free SPR biosensors for biomedical and agricultural applications. Towards this aim, SPR sensors with integrated microfluidic circuitry have been developed ultimately allowing a simplified on-site use.<br \/>\nTo ensure the detection specificity, this type of sensor requires a surface functionalization which is, in our case, based on either antibodies or DNA fragments. Two different pathogens, belonging to the fungal kingdom, namely Candida Albicans and Zymoseptoria Tritici, in the human and vegetal domains, respectively, were studied, detected, and quantified.<br \/>\nTo this end, the ligands of interest were first immobilised on the gold surface of the SPR sensors by amine coupling for the antibodies and by direct thiol-gold covalent bonding for the single stranded-DNA. Subsequently, biological interaction studies with the respective complementary antigens and DNA were performed in real time using two lab-made SPR systems; the first, which is fixed and based on angular interrogation and the second, which is portable and operating in spectral configuration scheme.<\/p>\n<\/div><\/section><\/div>","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":52863,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[8],"tags":[],"class_list":["post-52858","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-actualites"],"_links":{"self":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts\/52858","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/comments?post=52858"}],"version-history":[{"count":0,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts\/52858\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/media\/52863"}],"wp:attachment":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/media?parent=52858"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/categories?post=52858"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/tags?post=52858"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}