{"id":51466,"date":"2022-03-21T11:22:05","date_gmt":"2022-03-21T09:22:05","guid":{"rendered":"https:\/\/www.iemn.fr\/?p=51466"},"modified":"2022-05-09T15:49:09","modified_gmt":"2022-05-09T13:49:09","slug":"these-de-roudy-al-sahely-acoustical-tweezers-based-on-on-acoustical-vortices-for-3d-contactless-manipulation-of-microparticles-and-cells","status":"publish","type":"post","link":"https:\/\/www.iemn.fr\/en\/actualites\/these-de-roudy-al-sahely-acoustical-tweezers-based-on-on-acoustical-vortices-for-3d-contactless-manipulation-of-microparticles-and-cells.html","title":{"rendered":"Th\u00e8se de Roudy Al Sahely : \u201cAcoustical tweezers based on on acoustical vortices for 3D contactless manipulation of microparticles and cells\u201d"},"content":{"rendered":"<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-s5yax6-731f976cd073a09732242b81b12490ee\">\n.flex_column.av-s5yax6-731f976cd073a09732242b81b12490ee{\nborder-radius:0px 0px 0px 0px;\npadding:0px 0px 0px 0px;\n}\n<\/style>\n<div  class='flex_column av-s5yax6-731f976cd073a09732242b81b12490ee av_one_full  avia-builder-el-0  avia-builder-el-no-sibling  first flex_column_div av-zero-column-padding'     ><section  class='av_textblock_section av-l10i1idh-a1d9618c8775469b47d506d516ac2c70'   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><h4 style=\"text-align: center;\">\u00a0Th\u00e8se de Roudy Al Sahely<br \/>\n\u201cAcoustical tweezers based on on acoustical vortices<br \/>\nfor 3D contactless manipulation of microparticles and cells\u201d<\/h4>\n<blockquote><p>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-ikveov-92c0e1e8a70f730b34d6ee48b092e96b\">\n.av_font_icon.av-ikveov-92c0e1e8a70f730b34d6ee48b092e96b .av-icon-char{\nfont-size:30px;\nline-height:30px;\n}\n<\/style>\n<span  class='av_font_icon av-ikveov-92c0e1e8a70f730b34d6ee48b092e96b avia_animate_when_visible av-icon-style- avia-icon-pos-left av-no-color avia-icon-animate'><span class='av-icon-char' aria-hidden='true' data-av_icon='\ue8c9' data-av_iconfont='entypo-fontello' ><\/span><\/span>Soutenance le jeudi 31 mars \u00e0 13h45<br \/>\nAmphith\u00e9\u00e2tre de l\u2019IEMN \u2013 Laboratoire central \u2013 Villeneuve d\u2019Ascq<\/p>\n<\/blockquote>\n<h5>Jury :<\/h5>\n<p>Jean-Fran\u00e7ois Manceau, Professeur de l\u2019Universit\u00e9 de Franche-Comt\u00e9, Rapporteur<br \/>\nFr\u00e9d\u00e9ric Sarry, Professeur de l\u2019Universit\u00e9 de Lorraine, Rapporteur,<br \/>\nVirginie Ho\u00ebl, Professeur de l\u2019Universit\u00e9 de Lille, Examinateur,<br \/>\nMichael Baudoin, Professeur de l\u2019Universit\u00e9 de Lille, Co-directeur de th\u00e8se<br \/>\nOlivier Bou Matar, Professeur de l\u2019Universit\u00e9 de Lille, Co-directeur de th\u00e8se, Membre invit\u00e9<\/p>\n<h5>Summary:<\/h5>\n<p>Les pinces acoustiques bas\u00e9es sur les vortex acoustiques focalis\u00e9es ouvrent de nouvelles perspectives pour la manipulation sans contact et s\u00e9lective d\u2019objets millim\u00e9triques \u00e0 microm\u00e9triques, avec une s\u00e9lectivit\u00e9 et des forces appliqu\u00e9es difficilement atteignables avec les autres m\u00e9thodes. La premi\u00e8re d\u00e9monstration du pi\u00e9geage et de la manipulation 3D d\u2019une particule avec des vortex acoustiques a \u00e9t\u00e9 effectu\u00e9e en 2016 avec une matrice de transducteur ultrasonores pilot\u00e9s par une \u00e9lectronique programmable. R\u00e9cemment, il a \u00e9t\u00e9 propos\u00e9 d\u2019utiliser des pinces acoustiques holographiques bas\u00e9es sur des transducteurs interdigit\u00e9s en spirales pour d\u00e9velopper des pinces acoustiques miniaturis\u00e9es compatibles avec un environnement de microscopie standard.<br \/>\nDans cette th\u00e8se, nous avons explor\u00e9 les possibilit\u00e9s offertes par ce type de pince acoustiques pour adresser les probl\u00e8mes suivants. 1) Manipuler s\u00e9lectivement et organiser des cellules humaines avec des forces importantes (200 pN) sans pr\u00e9-marquage et sans affecter la viabilit\u00e9 des cellules. 2) Cr\u00e9er des pinces acoustiques tr\u00e8s haute fr\u00e9quence (250 MHz) avec une forte s\u00e9lectivit\u00e9 et capables de manipuler des microparticules de 4 microns avec des forces de l\u2019ordre du NanoNewton. 4) D\u00e9placer des microparticules en 3D dans un environnement libre et les translater axialement sans d\u00e9placement du transducteur. Ces objectifs ont \u00e9t\u00e9 atteints en d\u00e9veloppant de nouvelles m\u00e9thodes num\u00e9riques et des proc\u00e9dure exp\u00e9rimentales adapt\u00e9es, qui nous ont permis de concevoir des pinces acoustiques avec les capacit\u00e9s recherch\u00e9es. Ce travail ouvre des perspectives dans le domaine de la microbiologie pour \u00e9tudier les interactions cellulaires et leur r\u00e9ponse \u00e0 des sollicitations m\u00e9canique, mais aussi pour la spectroscopie de force acoustique.<\/p>\n<h5>Abstract:<\/h5>\n<p>Acoustical tweezers based on focused acoustical vortices open some tremendous perspectives for the in vitro and in vivo remote and selective manipulation of millimetric down to micrometric objects, with combined selectivity and applied forces out of reach with any other contactless manipulation technique. The first demonstration of 3D particle trapping and manipulation with acoustical vortices was achieved in 2016 with an array of transducers driven by programmable electronics. More recently it has been proposed to use holographic acoustical tweezers based on Archimedes-Fermat spiraling interdigitated transducers (S-IDTs) to design miniaturized acoustical tweezers compatible with a standard microscopy environment.<br \/>\nIn this PhD, we have explored the possibilities offered by these kinds of acoustical tweezers to address the following unsolved issues: 1) Manipulate selectively and organize human cells with large forces (200pN) without pre-tagging and without affecting the cells viability. 2) Create ultra-high frequency tweezers (250 MHz) with high spatial selectivity able to trap and position 4 microns individual microparticles with NanoNewton forces. 3) Manipulate microparticles in 3D in a free environment and translate them axially without motion of the transducer. These goals have been achieved by developing (i) a new numerical code based the combination of Finite Element simulation of the source and Angular Spectrum propagation of the wave and (ii) appropriate microfabrication procedures, which helped us design and fabricate tweezers with the good capabilities. This work open perspectives in microbiology to study cells interaction and their response to mechanical solicitation but also for acoustic forces spectroscopy.<\/p>\n<\/div><\/section><\/div>","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":12828,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[8],"tags":[],"class_list":["post-51466","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-actualites"],"_links":{"self":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts\/51466","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/comments?post=51466"}],"version-history":[{"count":0,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts\/51466\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/media\/12828"}],"wp:attachment":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/media?parent=51466"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/categories?post=51466"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/tags?post=51466"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}