{"id":43695,"date":"2020-12-14T18:20:55","date_gmt":"2020-12-14T16:20:55","guid":{"rendered":"https:\/\/www.iemn.fr\/?p=43695"},"modified":"2021-03-12T10:29:41","modified_gmt":"2021-03-12T08:29:41","slug":"these-de-sivaramakrishnan-ganesan-surface-plasmon-resonance-based-biosensor-applied-to-phytosanitary-domain","status":"publish","type":"post","link":"https:\/\/www.iemn.fr\/en\/actualites\/these-de-sivaramakrishnan-ganesan-surface-plasmon-resonance-based-biosensor-applied-to-phytosanitary-domain.html","title":{"rendered":"Thesis by Sivaramakrishnan Ganesan \u2013 Surface plasmon resonance based biosensor applied to phytosanitary domain"},"content":{"rendered":"<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-17023pr-61b8d2841f1052accf135e6b1e6b44c7\">\n.flex_column.av-17023pr-61b8d2841f1052accf135e6b1e6b44c7{\nborder-radius:0px 0px 0px 0px;\npadding:0px 0px 0px 0px;\n}\n<\/style>\n<div  class='flex_column av-17023pr-61b8d2841f1052accf135e6b1e6b44c7 av_one_full  avia-builder-el-0  avia-builder-el-no-sibling  first flex_column_div av-zero-column-padding'     ><section  class='av_textblock_section av-kiori78f-6e3c4f22740e8b0d73ce624d7fc12b6b'   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><h3 style=\"text-align: center;\">Soutenance de th\u00e8se de Sivaramakrishnan GANESAN<\/h3>\n<blockquote>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-w50llb-f10d1c3d5b70cd1fe53cd8fd2f1fa0fe\">\n.av_font_icon.av-w50llb-f10d1c3d5b70cd1fe53cd8fd2f1fa0fe .av-icon-char{\nfont-size:40px;\nline-height:40px;\n}\n<\/style>\n<span  class='av_font_icon av-w50llb-f10d1c3d5b70cd1fe53cd8fd2f1fa0fe avia_animate_when_visible av-icon-style- avia-icon-pos-left av-no-color avia-icon-animate'><span class='av-icon-char' aria-hidden='true' data-av_icon='\ue8c9' data-av_iconfont='entypo-fontello' ><\/span><\/span><\/blockquote>\n<blockquote>\n<p><strong>Mardi 15 d\u00e9cembre 2020 \u00e0 14h00<\/strong><\/p>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-w8y26n-90c5594a781c7163593e92a558496ba4\">\n.av_font_icon.av-w8y26n-90c5594a781c7163593e92a558496ba4 .av-icon-char{\nfont-size:20px;\nline-height:20px;\n}\n<\/style>\n<span  class='av_font_icon av-w8y26n-90c5594a781c7163593e92a558496ba4 avia_animate_when_visible av-icon-style- avia-icon-pos-left av-no-color avia-icon-animate'><span class='av-icon-char' aria-hidden='true' data-av_icon='\ue906' data-av_iconfont='entypo-fontello' ><\/span><\/span>\n<p><strong>La soutenance sera retransmise via deux supports<\/strong> :<br \/>\nDefense will be webstreamed on:<br \/>\n\u2013 slide view: Renavisio: http:\/\/desktop.visio.renater.fr\/scopia?ID=728617***5324&amp;autojoin<br \/>\n(Si vous vous connectez via Renavisio, pri\u00e8re de couper microphone et cam\u00e9ra ; people connecting via Renavisio are asked to switch off microphone and camera)<br \/>\n\u2013 room view: Youtube: https:\/\/youtu.be\/T9u9uHPB_24<\/p>\n<\/blockquote>\n<h5>Jury :<\/h5>\n<p>M. Serge HABRAKEN, Professeur, Universit\u00e9 de Li\u00e8ge, Rapporteur<\/p>\n<p>M. Gilles LERONDEL,\u00a0Professeur, Universit\u00e9 Technologique de Troyes, Rapporteur<\/p>\n<p>M. Fabian DORTU, Ing\u00e9nieur, MULTITEL (Mons), Examinateur<\/p>\n<p>M. Tuami LASRI, Professeur, Universit\u00e9 de Lille, Examinateur<\/p>\n<p>Mme Sophie MARICOT, Ma\u00eetre de Conf\u00e9rences, Universit\u00e9 de Lille,\u00a0Encadrant<\/p>\n<p>M. Jean-Pierre VILCOT, Directeur de Recherche, CNRS (Villeneuve d\u2019Ascq), Directeur de th\u00e8se<\/p>\n<h5>Summary:<\/h5>\n<p>Ce travail de th\u00e8se s\u2019inscrivait dans celui, plus g\u00e9n\u00e9ral, de d\u00e9veloppement d\u2019un syst\u00e8me de d\u00e9tection biologique portable affichant sensibilit\u00e9, pr\u00e9cision et r\u00e9p\u00e9tabilit\u00e9 et dont le champ d\u2019applications visait les domaines m\u00e9dicaux et phytosanitaires. Ce syst\u00e8me est bas\u00e9 sur le principe physique de base qui est la R\u00e9sonance Plasmonique de Surface. Le but \u00e9tait alors de d\u00e9mocratiser cette technique au niveau du chevet du patient ou du champ. Dans ce contexte et dans le cadre du projet INTERREG FWVL BIOSENS, je me suis plus particuli\u00e8rement attach\u00e9 \u00e0 l\u2019\u00e9tude de diff\u00e9rentes innovations au niveau du capteur. Parmi toutes les techniques de d\u00e9tection biologique, la technique de mesure \u00e0 base de SPR a d\u00e9j\u00e0 \u00e9t\u00e9 amplement \u00e9tudi\u00e9e car elle pr\u00e9sente des avantages ind\u00e9niables comme sa sensibilit\u00e9, sa mesure en temps r\u00e9el ou encore la non-n\u00e9cessit\u00e9 de marquage des mol\u00e9cules. Bien que cette technique soit donc bien connue dans le domaine de la d\u00e9tection, la temp\u00e9rature de tout \u00e9chantillon influe sur son indice optique, qui est la seule valeur d\u00e9tect\u00e9e par un tel capteur, et peut donc \u00eatre interpr\u00e9t\u00e9e comme le r\u00e9sultat d\u2019une interaction biologique. Ceci am\u00e8ne un r\u00e9el d\u00e9fi dans le cas d\u2019un syst\u00e8me portable pour lequel l\u2019int\u00e9gration d\u2019une enceinte thermostat\u00e9e est source d\u2019encombrement et de consommation \u00e9nerg\u00e9tique. Un capteur SPR 4 canaux a \u00e9t\u00e9 d\u00e9velopp\u00e9 et la temp\u00e9rature des \u00e9chantillons est directement contr\u00f4l\u00e9e par chauffage par effet Joule de la couche plasmonique elle-m\u00eame. Le fonctionnement de ce type de chauffage a \u00e9t\u00e9 mod\u00e9lis\u00e9 sous COMSOL et la caract\u00e9risation exp\u00e9rimentale a montr\u00e9 qu\u2019il ne perturbait pas le principe de d\u00e9tection. De l\u2019eau a \u00e9t\u00e9 chauff\u00e9e par injection de courant et la variation d\u2019indice optique obtenue compar\u00e9e \u00e0 la th\u00e9orie. Nous avons aussi d\u00e9montr\u00e9 qu\u2019il \u00e9tait possible de contr\u00f4ler en temp\u00e9rature ind\u00e9pendamment chacun des quatre canaux sans apparition d\u2019interf\u00e9rence thermique. De mani\u00e8re \u00e0 contr\u00f4ler plus finement la temp\u00e9rature, un capteur de temp\u00e9rature a aussi \u00e9t\u00e9 int\u00e9gr\u00e9 au niveau de la couche plasmonique. Ce contr\u00f4le de la temp\u00e9rature peut alors \u00eatre utilis\u00e9 soit pour maintenir une temp\u00e9rature constante au niveau des \u00e9chantillons soit pour d\u00e9terminer l\u2019effet de la temp\u00e9rature sur une interaction biologique. De mani\u00e8re \u00e0 diminuer le co\u00fbt du capteur, un capteur sur film plastique a \u00e9t\u00e9 fabriqu\u00e9 et test\u00e9. Il a permis d\u2019obtenir une r\u00e9ponse plasmonique sur l\u2019air et l\u2019eau. L\u2019utilisation d\u2019un prisme en PDMS ainsi que son couplage avec le capteur sur film a \u00e9t\u00e9 investigu\u00e9e de mani\u00e8re \u00e0 obtenir une plateforme de test la moins co\u00fbteuse possible.<\/p>\n<h5>Abstract:<\/h5>\n<p>The overall goal of this study was to develop a portable biological detection system with high sensitivity, accuracy and repeatability to be used in either the phytosanitary or the medical domain. This system is based on the Surface Plasmon Resonance (SPR) physical principle. Such a use of this principle is already achieved but leads to laboratory equipment. The goal here is to democratize its use up to an on field or point of care measurement system. In this context and within the frame of the BIOSENS INTEREG FWVL project, I worked more particularly on several developments around the sensor. From all the existing biosensor technologies, surface plasmon resonance (SPR) sensing technology has received continuous attention due to its advantages of a high-sensitivity, label-free and fast response time. Although the SPR sensing technique being legend in the sensor community, currently the temperature of the sample needs to be carefully maintained and controlled because SPR signal varies with temperature and any change in SPR signal can be interpreted as a biological interaction. This gives a huge challenge in the portable application that is targeted since embedding of a temperature controlled chamber size and energy consuming. A SPR sensor design including 4 SPR channels has been made and the temperature is controlled in real time by using Joule effect. Temperature behavior has been modelled under COMSOL and we experimentally demonstrated that the temperature modulation of SPR channel by Joule effect does not affect the detection scheme. Water was heated by injecting current through the plasmonic layer and the refractive index change of water due to temperature was measured and compared with the theory. We also demonstrated that the temperature of each of the 4 channels can be controlled independently without any thermal crosstalk. In order achieving a more precise monitoring of sample temperature, a temperature sensor was also fabricated on top of the plasmonic layer, allowing the real temperature at the sensor surface to be monitored in real time. This temperature control can so be used either to maintain the temperature of biologic samples or to identify the effect of temperature on biomolecular interactions. On addressing the cost of the sensing tool, a flexible and cheap SPR sensor made on tape has been fabricated and tested. Air and water plasmonic responses were measured using such a \u00ab\u00a0tape sensor\u00a0\u00bb. A PDMS prism has already been investigated and combined to the tape sensor making then a very cheap sensing platform.<\/p>\n<\/div><\/section><\/div>","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":43702,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[8,40],"tags":[],"class_list":["post-43695","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-actualites","category-articles-temporaires-anglais"],"_links":{"self":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts\/43695","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/comments?post=43695"}],"version-history":[{"count":0,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts\/43695\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/media\/43702"}],"wp:attachment":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/media?parent=43695"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/categories?post=43695"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/tags?post=43695"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}