{"id":42926,"date":"2020-10-01T13:52:27","date_gmt":"2020-10-01T11:52:27","guid":{"rendered":"https:\/\/www.iemn.fr\/articles-temporaires-anglais\/un-nouveau-type-de-microelectrodes-mea-pour-une-caracterisation-optique-et-electrique-dun-reseau-de-neurones-in-vitro-2.html"},"modified":"2020-11-24T17:12:42","modified_gmt":"2020-11-24T15:12:42","slug":"un-nouveau-type-de-microelectrodes-mea-pour-une-caracterisation-optique-et-electrique-dun-reseau-de-neurones-in-vitro-2","status":"publish","type":"post","link":"https:\/\/www.iemn.fr\/en\/newsletter\/un-nouveau-type-de-microelectrodes-mea-pour-une-caracterisation-optique-et-electrique-dun-reseau-de-neurones-in-vitro-2.html","title":{"rendered":"A new type of microelectrode (MEA) for optical and electrical characterization of a neural network in vitro."},"content":{"rendered":"<div id='layer_slider_1'  class='avia-layerslider main_color avia-shadow  avia-builder-el-0  el_before_av_one_full  avia-builder-el-first  container_wrap 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https:\/\/www.iemn.fr\/wp-content\/uploads\/2018\/11\/sliders_groupe_acoustique-768x80.jpg 768w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2018\/11\/sliders_groupe_acoustique-1030x107.jpg 1030w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2018\/11\/sliders_groupe_acoustique-1500x156.jpg 1500w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2018\/11\/sliders_groupe_acoustique-705x73.jpg 705w\" sizes=\"auto, (max-width: 2600px) 100vw, 2600px\" \/><\/div><\/div><\/div><div id='after_layer_slider_1'  class='main_color av_default_container_wrap container_wrap sidebar_right'  ><div class='container av-section-cont-open' ><div class='template-page content  av-content-small alpha units'><div class='post-entry post-entry-type-page post-entry-42926'><div class='entry-content-wrapper clearfix'>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-ih6dwm-c0390045fae7b0f07b14d1ec64d1f818\">\n.flex_column.av-ih6dwm-c0390045fae7b0f07b14d1ec64d1f818{\nborder-radius:0px 0px 0px 0px;\npadding:0px 0px 0px 0px;\n}\n<\/style>\n<div  class='flex_column av-ih6dwm-c0390045fae7b0f07b14d1ec64d1f818 av_one_full  avia-builder-el-1  el_after_av_layerslider  el_before_av_one_full  avia-builder-el-first  first flex_column_div av-zero-column-padding'     ><section  class='av_textblock_section av-kfqs577w-b7c3550467f47dce013a812928e5cad4'   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><h3 style=\"text-align: center;\"><em>A new type of microelectrode (MEA) for optical and electrical characterization of a neural network in vitro.<br \/>\n<\/em><\/h3>\n<hr \/>\n<h3 style=\"text-align: center;\"><\/h3>\n<\/div><\/section><\/div>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-ikoudi-ce719c7151c34a2a581a30a09f49589c\">\n.flex_column.av-ikoudi-ce719c7151c34a2a581a30a09f49589c{\nborder-radius:0px 0px 0px 0px;\npadding:0px 0px 0px 0px;\n}\n<\/style>\n<div  class='flex_column av-ikoudi-ce719c7151c34a2a581a30a09f49589c av_one_full  avia-builder-el-3  el_after_av_one_full  avia-builder-el-last  first flex_column_div av-zero-column-padding  column-top-margin'     ><section  class='av_textblock_section av-kfqrakvy-93df2165636767a713382d76673ffecb'   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><blockquote>\n<p>A collaboration involving several researchers from different groups at IEMN (NCM, NBI, BioMEMS, Carbon and NAM6) as well as the LilNCog center (formerly JPARC) has led to the development of a new type of microelectrode array (MicroElectrodesArrays \u2013 MEA).<br \/>\nRealized in PEDOT:PSS, they are totally transparent and allow to have an electrophysiological measurement system combined with an optical reading for the characterization of neural networks in vitro.<br \/>\nThe targeted applications extend to calcium imaging, immunomarking and optogenetics.<\/p>\n<\/blockquote>\n<p>Microelectrode arrays (MEAs) are used to record the in vitro biological activity of neuronal cells, heart cells or brain slices. However, most commercially available MEA systems incorporate non-transparent electrodes. Recently, the development of integrated measurement tools combining both electro- and optophysiology has been of great interest for a better understanding of physiological phenomena in neural networks. The development of transparent microelectrodes allows simultaneous electrophysiological and optical imaging measurements. Some devices have already been developed and even marketed. They use ITO or graphene electrodes, but always to the detriment of impedance values (too high) or transparency (too low). A compromise is systematically necessary.<\/p>\n<p>In this study, we developed optically transparent MEAs entirely made of PEDOT:PSS. They have low electrical impedance values (33 M\u03a9 \u00b5m2), comparable to the impedance values of commercially opaque MEA devices (TiN electrodes). This new type of MEA thus paves the way for many cutting-edge applications in neuroscience: exploration of the physiological state of neuronal cells under normal or stressful conditions, study of the complexity and cellular heterogeneity of the neural network under consideration, and to perform optomodulation experiments (activation\/inactivation of certain functions) of neural networks, such as optogenetic and photochemical modulation (controlled drug release).<\/p>\n<p><em>To carry out this work, researchers from the IEMN and the LilNCog center received support from a PEPS project (M2NP), the DISTAlZ lab and the ERC CoG Ionos.<\/em><\/p>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-13ewzjw-48aa122f75f0ed1ff23062fbde8d543c\">\n.av_font_icon.av-13ewzjw-48aa122f75f0ed1ff23062fbde8d543c{\ncolor:#bfbfbf;\nborder-color:#bfbfbf;\n}\n.av_font_icon.av-13ewzjw-48aa122f75f0ed1ff23062fbde8d543c .av-icon-char{\nfont-size:60px;\nline-height:60px;\n}\n<\/style>\n<span  class='av_font_icon av-13ewzjw-48aa122f75f0ed1ff23062fbde8d543c avia_animate_when_visible av-icon-style- avia-icon-pos-left avia-icon-animate'><span class='av-icon-char' aria-hidden='true' data-av_icon='\ue803' data-av_iconfont='entypo-fontello' ><\/span><\/span>\n<p><strong>Learn more:<\/strong><br \/>\n<a href=\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S0925400520312429?via%3Dihub\" target=\"_blank\" rel=\"noopener noreferrer\"><strong><span style=\"color: #800000;\">https:\/\/authors.elsevier.com\/a\/1bpq83IQMPISPj<\/span><\/strong><\/a><\/p>\n<div  class='avia-button-wrap av-rpqvoq-fdd1a586a19f3a30fac48f667bb61fd5-wrap avia-button-left  avia-builder-el-6  el_after_av_font_icon  avia-builder-el-last'><a href='mailto:yannick.coffinier@univ-lille.fr'  class='avia-button av-rpqvoq-fdd1a586a19f3a30fac48f667bb61fd5 av-link-btn avia-icon_select-yes-left-icon avia-size-small avia-position-left avia-color-silver'   aria-label=\"yannick.coffinier@univ-lille.fr\"><span class='avia_button_icon avia_button_icon_left' aria-hidden='true' data-av_icon='\ue805' data-av_iconfont='entypo-fontello'><\/span><span class='avia_iconbox_title' >yannick.coffinier@univ-lille.fr<\/span><\/a><\/div>\n<p><a href=\"https:\/\/www.iemn.fr\/wp-content\/uploads\/2020\/10\/visuel2.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-42600 size-full\" src=\"https:\/\/www.iemn.fr\/wp-content\/uploads\/2020\/10\/visuel2.jpg\" alt=\"\" width=\"613\" height=\"707\" srcset=\"https:\/\/www.iemn.fr\/wp-content\/uploads\/2020\/10\/visuel2.jpg 613w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2020\/10\/visuel2-260x300.jpg 260w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2020\/10\/visuel2-611x705.jpg 611w\" sizes=\"auto, (max-width: 613px) 100vw, 613px\" \/><\/a><\/p>\n<\/div><\/section><\/div>","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":42620,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[297],"tags":[],"class_list":["post-42926","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-newsletter"],"_links":{"self":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts\/42926","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/comments?post=42926"}],"version-history":[{"count":0,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts\/42926\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/media\/42620"}],"wp:attachment":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/media?parent=42926"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/categories?post=42926"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/tags?post=42926"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}