{"id":34603,"date":"2019-01-30T10:11:21","date_gmt":"2019-01-30T08:11:21","guid":{"rendered":"https:\/\/www.iemn.fr\/articles-temporaires\/these-2-4.html"},"modified":"2019-03-11T12:26:18","modified_gmt":"2019-03-11T10:26:18","slug":"these-2-4","status":"publish","type":"post","link":"https:\/\/www.iemn.fr\/en\/these\/these-2-4.html","title":{"rendered":"\u00c9tude par microscopie \u00e0 sonde locale des propri\u00e9t\u00e9s \u00e9lectriques de monocouches auto-assembl\u00e9es de mol\u00e9cules photo-commutables sur substrats ferromagn\u00e9tiques"},"content":{"rendered":"<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-av_heading-a927e79c498246c57d4b75a7b62731c5\">\n#top .av-special-heading.av-av_heading-a927e79c498246c57d4b75a7b62731c5{\npadding-bottom:10px;\n}\nbody .av-special-heading.av-av_heading-a927e79c498246c57d4b75a7b62731c5 .av-special-heading-tag .heading-char{\nfont-size:25px;\n}\n.av-special-heading.av-av_heading-a927e79c498246c57d4b75a7b62731c5 .av-subheading{\nfont-size:15px;\n}\n<\/style>\n<div  class='av-special-heading av-av_heading-a927e79c498246c57d4b75a7b62731c5 av-special-heading-h3'><h3 class='av-special-heading-tag'  itemprop=\"headline\"  >THESIS: Cell death initiated by singlet oxygen: evidence of long-range effects<\/h3><div class=\"special-heading-border\"><div class=\"special-heading-inner-border\"><\/div><\/div><\/div>\n<div  class='hr av-15ollfj-7e5f61e7db46264ca176bbad8d8d39ef hr-default'><span class='hr-inner'><span class=\"hr-inner-style\"><\/span><\/span><\/div>\n<section  class='av_textblock_section av-jriy64i8-2f4600354c0449b610997916bbd9b6bc'   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><blockquote>\n<p><strong><a href=\"https:\/\/www.iemn.fr\/wp-content\/uploads\/2018\/03\/ico_these2.fw_.png\"><img loading=\"lazy\" decoding=\"async\" class=\"size-thumbnail wp-image-14684 alignleft\" src=\"https:\/\/www.iemn.fr\/wp-content\/uploads\/2018\/03\/ico_these2.fw_-80x80.png\" alt=\"\" width=\"80\" height=\"80\" srcset=\"https:\/\/www.iemn.fr\/wp-content\/uploads\/2018\/03\/ico_these2.fw_-80x80.png 80w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2018\/03\/ico_these2.fw_-36x36.png 36w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2018\/03\/ico_these2.fw_-180x180.png 180w\" sizes=\"auto, (max-width: 80px) 100vw, 80px\" \/><\/a>\u00a0H\u00e9l\u00e8ne MOULET<\/strong><\/p>\n<p><strong>Thesis defence<\/strong><br \/>\n<strong>Tuesday 05 February 2019 at 2.00 pm<\/strong><br \/>\n<strong>Campus Cit\u00e9 Scientifique, B\u00e2t. P5, Amphith\u00e9\u00e2tre Pierre Glorieux\u00a0<\/strong><\/p>\n<\/blockquote>\n<p><strong><br \/>\nJury:<\/strong><\/p>\n<ul>\n<li><span class=\"\">St\u00e9phanie BONNEAU (Pierre and Marie Curie University, Rapporteur), <\/span><\/li>\n<li><span class=\"\">C\u00e9lineFROCHOTt (University of Lorraine, Rapporteur)<\/span><\/li>\n<li><span class=\"\">Peter OGILBY (University of Aarhus, Examiner)<\/span><\/li>\n<li><span class=\"\">Marc DOUAY (University of Lille, Examiner) <\/span><\/li>\n<li>Emmanuel COURTADE (University of Lille, thesis supervisor)<\/li>\n<li>Anthony TREIZEBRE (University of Lille, co-supervisor)<\/li>\n<\/ul>\n<p><strong>\u00a0R\u00e9sum\u00e9:<\/strong><br \/>\n<span class=\"\">Singlet oxygen (1O2), the first excited electronic state of oxygen, is the major cytotoxic agent in photodynamic therapy. We used direct optical activation of dioxygen to establish a quantitative link between the rate of 1O2 production and cell death.<br \/>\n<\/span><\/p>\n<p><span class=\"\">In tumour spheroids, which reproduce the geometry of tumours in vitro, we demonstrate long-range cell death that cannot be explained by the direct action of 1O2. This death is due to 1O2 produced within the spheroids but outside the cells. We have set up an experiment that allows us to spatially control the production of 1O2 outside the cells. The cell death observed at long range in these experiments involves the presence of secondary reactive oxygen species. <\/span><\/p>\n<p><span class=\"\">Finally, certain types of death are preferred from a therapeutic point of view, in order to limit the inflammatory response, among other things. We have set up an in vitro experiment that allows us to observe different modes of death as a function of the rate of 1O2 production and the exposure time.<\/span><\/p>\n<p>Abstract:<br \/>\n<span class=\"\">Singlet oxygen (1O2) is the first excited state of molecular oxygen. It is the major cytotoxic agent in photo dynamic therapy. We use direct optical excitation of oxygen to quantitatively estimate 1O2 production rate in cells and to study its cytotoxic effects.<\/span><\/p>\n<p><span class=\"\">In multicellular tumor spheroids, which mimic tumor geometry in vitro, we highlight longrange cell death that cannot be explained by singlet oxygen alone. This death is caused by 1O2 generated within spheroids but outside of the cells. We set up an experiment enabling spatial control of extra-cellular 1O2 production. The measured long-range cell death in these experiments implies the presence of secondary reactive oxygen species.<\/span><\/p>\n<p><span class=\"\">Lastly, some cell death modalities are preferred from a treatment perspective, in order, for example, to limit inflammatory response. We set up an experiment that enabled us to observe different cell death modalities according to 1O2 production rates and exposure times.<\/span><strong><br \/>\n<\/strong><\/p>\n<\/div><\/section>","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[204,199],"tags":[139],"class_list":["post-34603","post","type-post","status-publish","format-standard","hentry","category-maj-slider","category-these","tag-these"],"_links":{"self":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts\/34603","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/comments?post=34603"}],"version-history":[{"count":0,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/posts\/34603\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/media?parent=34603"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/categories?post=34603"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.iemn.fr\/en\/wp-json\/wp\/v2\/tags?post=34603"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}