{"id":74709,"date":"2025-07-21T11:51:05","date_gmt":"2025-07-21T09:51:05","guid":{"rendered":"https:\/\/www.iemn.fr\/?page_id=74709"},"modified":"2026-03-16T16:52:14","modified_gmt":"2026-03-16T14:52:14","slug":"anr-g-virals","status":"publish","type":"page","link":"https:\/\/www.iemn.fr\/en\/anr-g-virals","title":{"rendered":"Projet ANR : D\u00e9tection de RNA double brin sur transistors \u00e0 effet de champ \u00e0 base de graph\u00e8ne : une nouvelle voie pour la surveillance virale"},"content":{"rendered":"<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-obgwup-e849561b42eb2866e0aa5229228349dd\">\n.flex_column.av-obgwup-e849561b42eb2866e0aa5229228349dd{\nborder-radius:0px 0px 0px 0px;\npadding:0px 0px 0px 0px;\n}\n<\/style>\n<div  class='flex_column av-obgwup-e849561b42eb2866e0aa5229228349dd av_one_third  avia-builder-el-0  el_before_av_one_third  avia-builder-el-first  first flex_column_div av-zero-column-padding'     ><\/div>\n\n<style 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itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\" ><div class=\"avia-image-container-inner\"><div class=\"avia-image-overlay-wrap\"><img decoding=\"async\" fetchpriority=\"high\" class='wp-image-53098 avia-img-lazy-loading-not-53098 avia_image' src=\"https:\/\/www.iemn.fr\/wp-content\/uploads\/2022\/06\/logo_anr.jpg\" alt='' title='logo_anr'  height=\"85\" width=\"300\"  itemprop=\"thumbnailUrl\" srcset=\"https:\/\/www.iemn.fr\/wp-content\/uploads\/2022\/06\/logo_anr.jpg 300w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2022\/06\/logo_anr-18x5.jpg 18w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/div><\/div><\/div><\/div>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-obgwup-12-aafefada72b62fbdec152f43fe69b335\">\n.flex_column.av-obgwup-12-aafefada72b62fbdec152f43fe69b335{\nborder-radius:0px 0px 0px 0px;\npadding:0px 0px 0px 0px;\n}\n<\/style>\n<div  class='flex_column av-obgwup-12-aafefada72b62fbdec152f43fe69b335 av_one_third  avia-builder-el-3  el_after_av_one_third  el_before_av_one_full  flex_column_div av-zero-column-padding'     ><\/div>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-l48hejsx-e4793490ff2a2a809d51b2d1d36f4496\">\n.flex_column.av-l48hejsx-e4793490ff2a2a809d51b2d1d36f4496{\nborder-radius:20px 20px 20px 20px;\npadding:10px 10px 10px 10px;\nbackground-color:#ebb401;\n}\n<\/style>\n<div  class='flex_column av-l48hejsx-e4793490ff2a2a809d51b2d1d36f4496 av_one_full  avia-builder-el-4  el_after_av_one_third  el_before_av_one_full  first flex_column_div  column-top-margin'     ><section  class='av_textblock_section av-l48hdtga-50380e38c85ad2e1915c9650e85ebb6d'   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock'  itemprop=\"text\" ><h3 style=\"text-align: center;\">Projet G- VIRALS ANR-23-GRF1-0004<\/h3>\n<\/div><\/section><\/div>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-lkgiy9-6fe9252508a87bef590816866096ff99\">\n.flex_column.av-lkgiy9-6fe9252508a87bef590816866096ff99{\nborder-radius:0px 0px 0px 0px;\npadding:20px 0px 20px 0px;\n}\n<\/style>\n<div  class='flex_column av-lkgiy9-6fe9252508a87bef590816866096ff99 av_one_full  avia-builder-el-6  el_after_av_one_full  el_before_av_textblock  first flex_column_div  column-top-margin'     ><section  class='av_textblock_section av-l48g5n3e-1551bed9ef7e820e4f83d8e2482ed0e9'   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock'  itemprop=\"text\" ><h4>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-mqde7m-b8f4c22e6bbeb9225144fbb1e9b8c6d8\">\n.av_font_icon.av-mqde7m-b8f4c22e6bbeb9225144fbb1e9b8c6d8{\ncolor:#ebb401;\nborder-color:#ebb401;\n}\n.av_font_icon.av-mqde7m-b8f4c22e6bbeb9225144fbb1e9b8c6d8 .av-icon-char{\nfont-size:25px;\nline-height:25px;\n}\n<\/style>\n<span  class='av_font_icon av-mqde7m-b8f4c22e6bbeb9225144fbb1e9b8c6d8 avia_animate_when_visible av-icon-style- avia-icon-pos-left avia-icon-animate'><span class='av-icon-char' aria-hidden='true' data-av_icon='\ue889' data-av_iconfont='entypo-fontello' ><\/span><\/span>G-VIRALS<\/h4>\n<h5><\/h5>\n<h5><span id=\"page133R_mcid13\" class=\"markedContent\">\u00a0<\/span><\/h5>\n<\/div><\/section><\/div>\n<section  class='av_textblock_section av-l48g64e5-d7772cf38373e1395e19db96febaa039'   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock'  itemprop=\"text\" ><ul>\n<li><strong><span style=\"color: #800000;\">IEMN Project Sponsor:<\/span> <span id=\"page133R_mcid14\" class=\"markedContent\"><span dir=\"ltr\" role=\"presentation\">Henri Happy<\/span><\/span> <\/strong><\/li>\n<li><strong><span style=\"color: #800000;\">Coordinator:<\/span> IEMN<br \/>\n<\/strong><\/li>\n<li><strong><span style=\"color: #800000;\">Partners:<\/span> <\/strong>IBMP Institut de biologie mol\u00e9culaire des plantes (UPR 2357)<br \/>\nITODYS Interfaces, Traitements, Organisation et Dynamique des Syst\u00e8mes<br \/>\nIEMN UMR 8520 &#8211; IEMN &#8211; Institut d&rsquo;Electronique, de Micro\u00e9lectronique et de Nanotechnologie<br \/>\nUniversity of Regensburg University of Regensburg<br \/>\nUCLouvain Universit\u00e9 catholique de Louvain<br \/>\nCentre for Adv. Materials Application Centre for Advanced Materials Application<\/li>\n<\/ul>\n<p><strong><u>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-13ewzjw-b58072c982babce8ed7840ecc9331fc9\">\n.av_font_icon.av-13ewzjw-b58072c982babce8ed7840ecc9331fc9{\ncolor:#800000;\nborder-color:#800000;\n}\n.av_font_icon.av-13ewzjw-b58072c982babce8ed7840ecc9331fc9 .av-icon-char{\nfont-size:20px;\nline-height:20px;\n}\n<\/style>\n<span  class='av_font_icon av-13ewzjw-b58072c982babce8ed7840ecc9331fc9 avia_animate_when_visible av-icon-style- avia-icon-pos-left avia-icon-animate'><span class='av-icon-char' aria-hidden='true' data-av_icon='\ue885' data-av_iconfont='entypo-fontello' ><\/span><\/span><\/u><span style=\"color: #800000;\">Abstract:<\/span><\/strong><\/p>\n<p>Viruses are small intracellular parasites, which by definition contain either a RNA or DNA genome surrounded by a protective structure consisting of proteins and additional lipids when enveloped. These parasites are very diverse in shape, size, composition and hosts covering the entire living world. Due to this diversity, the development of a simple and \u201cgeneral approach\u201d for virus detection remains thus highly challenging. While polymerase chain reaction (PCR) and reverse transcription-PCR (RT-PCR) have become the gold standards for the identification and quantification of viruses, these methods are targeted and require prior knowledge of the specific virus to be detected similarly to antigen-based methods such as ELISA. PCR for the detection can also challenging due to the propensity of viruses to mutate at high frequency.<\/p>\n<p>Long viral double-stranded RNA (dsRNA) is considered a universal biomarker for the presence of virus and a unique hallmark of many viral infections. dsRNA is produced either by RNA viruses during replication or by DNA viruses upon convergent transcription. Thus, unlike s<a href=\"https:\/\/www.iemn.fr\/wp-content\/uploads\/2025\/07\/happy.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignright size-full wp-image-74722\" src=\"https:\/\/www.iemn.fr\/wp-content\/uploads\/2025\/07\/happy.jpg\" alt=\"\" width=\"724\" height=\"507\" srcset=\"https:\/\/www.iemn.fr\/wp-content\/uploads\/2025\/07\/happy.jpg 724w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2025\/07\/happy-300x210.jpg 300w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2025\/07\/happy-18x12.jpg 18w, https:\/\/www.iemn.fr\/wp-content\/uploads\/2025\/07\/happy-705x494.jpg 705w\" sizes=\"auto, (max-width: 724px) 100vw, 724px\" \/><\/a>ingle-stranded RNA and short RNA duplexes like siRNA, the presence of dsRNA &gt;40 base pairs in the cytoplasm of cells is generally a sign of viral infection and triggers innate immune responses. Detection of these long dsRNA is currently based on time-consuming and costly immunofluorescence using dsRNA specific antibodies.<\/p>\n<p>Paradoxically, dsRNA-centred viral surveillance strategies have not yet inspired widespread research initiatives. We believe this is partially due to the lack of deeper knowledge about the dynamic viral RNA-protein interactions, essential to understanding their evolution and adaptation to distinct hosts and environments, and critical for developing virus surveillance sensors.<\/p>\n<p>G-Virals aims to overcome these challenges and knowledge gaps by focusing on the extraordinary dsRNA binding properties of proteins such as B2. Integration of dsRNA binding proteins into a panel of differently constructed graphene-based field effect transistors (gFETs) and connection to lab-on-chip based pre-analytical steps will allow quantification of viral dsRNA in a point-of-need sensing platform. Next to studying the key operational characteristics of this novel virus warning systems, flash Joule heating of biomedical wait will prepare \u201cflash\u201d graphene inks for gFET designs in parallel. G-Virals proposes the first generic approach for viral surveillance via the development of dsRNA-to-graphene. Implementation of precision qRT-PCR and third-generation sequencing (e.g., nanopore technology) in case of a positive sensor responses, will further result in virus identification. G-Virals will strongly impact responses to future and emerging viral diseases via the proposed systems of virus surveillance.<\/p>\n<\/div><\/section>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-lkgiy9-11-e733f3040143fc6ad9c0b5db21c9fa85\">\n.flex_column.av-lkgiy9-11-e733f3040143fc6ad9c0b5db21c9fa85{\nborder-radius:0px 0px 0px 0px;\npadding:20px 0px 20px 0px;\n}\n<\/style>\n<div  class='flex_column av-lkgiy9-11-e733f3040143fc6ad9c0b5db21c9fa85 av_one_full  avia-builder-el-11  el_after_av_textblock  el_before_av_one_fourth  first flex_column_div  column-top-margin'     ><section  class='av_textblock_section av-l48g5n3e-10-f123be668bfa1041a5b045bc8469811f'   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock'  itemprop=\"text\" ><h4>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-mqde7m-9-ba3319396eed72cd5acaa8015aeeda90\">\n.av_font_icon.av-mqde7m-9-ba3319396eed72cd5acaa8015aeeda90{\ncolor:#ebb401;\nborder-color:#ebb401;\n}\n.av_font_icon.av-mqde7m-9-ba3319396eed72cd5acaa8015aeeda90 .av-icon-char{\nfont-size:25px;\nline-height:25px;\n}\n<\/style>\n<span  class='av_font_icon av-mqde7m-9-ba3319396eed72cd5acaa8015aeeda90 avia_animate_when_visible av-icon-style- avia-icon-pos-left avia-icon-animate'><span class='av-icon-char' aria-hidden='true' data-av_icon='\ue889' data-av_iconfont='entypo-fontello' ><\/span><\/span>Partners<\/h4>\n<h5><\/h5>\n<\/div><\/section><\/div>\n<div class='flex_column_table av-237o9w1-e31f98e4cf06d668c03c0d3978576c28 sc-av_one_fourth av-equal-height-column-flextable'>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-237o9w1-e31f98e4cf06d668c03c0d3978576c28\">\n@keyframes av_boxShadowEffect_av-237o9w1-e31f98e4cf06d668c03c0d3978576c28-column {\n0%   { box-shadow:  0 0 0 0 #d3d3d3; 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